Discrete genetic loci in human gutBacteroides thetaiotaomicron confer pectin metabolism

doi:10.1016/j.carbpol.2021.118534

	Discrete genetic loci in human gutBacteroides thetaiotaomicron confer pectin metabolism
	Li, Meixia 1; Li, Saijuan 1; Guo, Xiaozhen 1; Guo, Ciliang 1; Wang, Yeqin 1; Du, Zhenyun 1; Zhang, Zhenqing 3,4; Xie, Cen 1,2; Ding, Kan 1,2
刊名	CARBOHYDRATE POLYMERS
	2021-11-15
卷号	272 页码:13
关键词	Polysaccharide Pectin Human gut microbiota Metabolism Bacteroides
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2021.118534
通讯作者	Xie, Cen(xiecen@simm.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn)
英文摘要	Although the polysaccharide utilization loci (PULs) activated by pectin have been defined, due to the complex of side-chain structure, the degradative mechanisms still remain vague. Thus, we hypothesize that there may have other specific PULs to target pectin. Here, we characterize loci-encoded proteins expressed by Bacteroides thetaiotaomicron (BT) that are involved in the pectin capturing, importation, de-branching and degradation into monosaccharides. Totally, four PULs contain ten enzymes and four glycan binding proteins which including a novel surface enzyme and a surface glycan binding protein are identified. Notably, PUL2 and PUL3 have not been reported so far. Further, we show that the degradation products support the growth of other Bacteroides spp. and probiotics. In addition, genes involved in this process are conservative in other Bacteroides spp. Our results further highlight the contribution of Bacteroides spp. to metabolism the pectic network.
资助项目	National Natural Science Foundation of China[31800673] ; Key New Drug Creation andManufacturing Program[2019ZX09735001] ; National Key Research and Development Program of China, Ministry of Science and Technology[2019YFC1711-000]
WOS关键词	POLYSACCHARIDE UTILIZATION ; GUT MICROBIOTA ; CATABOLISM ; GALECTIN-3 ; DIVERSITY ; EVOLUTION
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000689507000007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/297741]
专题	中国科学院上海药物研究所
通讯作者	Xie, Cen; Ding, Kan
作者单位	1.Chinese Acad Sci, Glycochem & Glycobiol Lab, Key Lab Receptor Res, Shanghai Inst Mat Med, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Soochow Univ, Jiangsu Key Lab Neuropsychiat Dis, Suzhou 215123, Jiangsu, Peoples R China 4.Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Li, Meixia,Li, Saijuan,Guo, Xiaozhen,et al. Discrete genetic loci in human gutBacteroides thetaiotaomicron confer pectin metabolism[J]. CARBOHYDRATE POLYMERS,2021,272:13.
APA	Li, Meixia.,Li, Saijuan.,Guo, Xiaozhen.,Guo, Ciliang.,Wang, Yeqin.,...&Ding, Kan.(2021).Discrete genetic loci in human gutBacteroides thetaiotaomicron confer pectin metabolism.CARBOHYDRATE POLYMERS,272,13.
MLA	Li, Meixia,et al."Discrete genetic loci in human gutBacteroides thetaiotaomicron confer pectin metabolism".CARBOHYDRATE POLYMERS 272(2021):13.