Screening of chemicals with binding activities of liver X receptors from reclaimed waters

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	Screening of chemicals with binding activities of liver X receptors from reclaimed waters
	Zhang, Haifeng; Jia, Yingting; Tang, Zhuoheng; Wang, Lei; Hu, Wenxin; Gao, Junmin; Hu, Jianying; Yang, Min
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2020-04-15
卷号	713 页码:1-59
关键词	Endocrine disrupting chemicals Liver X receptors Reclaimed wastewater Nuclear receptors binding extraction High resolution mass spectrometry
ISSN号	0048-9697
英文摘要	Wastewater reclamation and reuse is considered an attractive and practical method for coping with water scarcity. However, the presence of micropollutants in reclaimed water, including endocrine disrupting chemicals (EDCs), is a major public health concern. This study attempted to identify unknown EDCs with liver X receptor (LXR alpha) agonist/antagonist activities in reclaimed wastewater, using nuclear receptors binding extraction coupled with high-resolution mass spectrometry (NRBE-HRMS). In total, 105 compounds in the reclaimed wastewater exhibited LXR alpha-binding activity. Among them, two previously unknown LXR alpha-antagonist compounds, catechol and 4-acetamidoantipyrine, were identified, based on authentic standards. The two LXR alpha-antagonist compounds exhibited weak LXR alpha-antagonist activities in a yeast two-hybrid assay. Catechol and 4-acetamidoantipyrine inhibited the beta-galactosidase activity induced by 60 nM of TO901317 in an LXR alpha yeast assay, with IC20 values of 79,938.9 nM and 6286.4 nM, respectively. To the best of our knowledge, this is the first study to identify EDCs in reclaimed wastewater with LXR alpha-agonist/antagonist activity using the NRBE-HRMS method. (C) 2020 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/45360]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
推荐引用方式 GB/T 7714	Zhang, Haifeng,Jia, Yingting,Tang, Zhuoheng,et al. Screening of chemicals with binding activities of liver X receptors from reclaimed waters[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,713:1-59.
APA	Zhang, Haifeng.,Jia, Yingting.,Tang, Zhuoheng.,Wang, Lei.,Hu, Wenxin.,...&Yang, Min.(2020).Screening of chemicals with binding activities of liver X receptors from reclaimed waters.SCIENCE OF THE TOTAL ENVIRONMENT,713,1-59.
MLA	Zhang, Haifeng,et al."Screening of chemicals with binding activities of liver X receptors from reclaimed waters".SCIENCE OF THE TOTAL ENVIRONMENT 713(2020):1-59.