Effects of graphene oxide on PCR amplification for microbial community survey

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Effects of graphene oxide on PCR amplification for microbial community survey
	Li, Shuzhen; Wang, Zhujun; Wang, Yuanyuan; Song, Maoyong; Lu, Guangxin; Dang, Ning; Yin, Huaqun; Qu, Yuanyuan; Deng, Ye
刊名	BMC MICROBIOLOGY
	2020-09-11
卷号	20 期号:1 页码:1-11
关键词	Oxidized graphene Bacteria Fungi Chimera Community composition
ISSN号	1471-2180
英文摘要	Background Graphene oxide (GO) has been suggested as an efficient assistant additive to eliminate non-specific amplification of the polymerase chain reaction (PCR). Although many studies have focused on exploring its molecular mechanism, the practice of GO on the quantitation of microbial community has not been implemented yet. In this study, GO was added in PCR system to explore the changes on removing typical amplification errors, such as chimera and mismatches on two kinds of mock communities (an evenly mixed and a staggered mock communities) and environmental samples. Results High-throughput sequencing of bacterial and fungal communities, based on 16S rRNA genes and internal transcribed spacers (ITS) respectively, showed that GO could significantly increase large segmental error (chimeric sequence) in PCR procedure while had no specific effect on point error (mismatched sequence). Besides, GO reduced the alpha-diversity of community, and changed the composition of fungal community more obviously than bacterial community. Conclusions Our study provides the first quantitative data on microbial community level to prove the negative effect of GO, and also indicates that there may be a more complex interaction between GO and comprehensive DNA fragments in PCR process.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/45160]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Li, Shuzhen,Wang, Zhujun,Wang, Yuanyuan,et al. Effects of graphene oxide on PCR amplification for microbial community survey[J]. BMC MICROBIOLOGY,2020,20(1):1-11.
APA	Li, Shuzhen.,Wang, Zhujun.,Wang, Yuanyuan.,Song, Maoyong.,Lu, Guangxin.,...&Deng, Ye.(2020).Effects of graphene oxide on PCR amplification for microbial community survey.BMC MICROBIOLOGY,20(1),1-11.
MLA	Li, Shuzhen,et al."Effects of graphene oxide on PCR amplification for microbial community survey".BMC MICROBIOLOGY 20.1(2020):1-11.