A combination of mitochondrial DNA markers Ckmito and ND5-CD is recommended as the most reliable indicator for microbial source tracking to identify faecal pollution from poultry in China

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	A combination of mitochondrial DNA markers Ckmito and ND5-CD is recommended as the most reliable indicator for microbial source tracking to identify faecal pollution from poultry in China
	Liang, Hongxia; Yu, Zhisheng; Ndayisenga, Fabrice; Liu, Ruyin; Zhang, Yiming; Zhang, Hongxun; Wu, Gang
刊名	ECOLOGICAL INDICATORS
	2020-08
卷号	115 页码:1-10
关键词	Microbial source tracking Faecal indicators Poultry-associated markers Avian-associated markers Quantitative PCR
ISSN号	1470-160X
英文摘要	A suitable marker for microbial source tracking (MST) remains difficult to find in China due to the geographical variability of markers. 162 faecal and litter samples were collected from five different regions in China to identify the faecal pollution from poultry with nine published molecular markers for MST. All samples tested were positive for both universal markers BacUni and MI50, but BacUni was detected with higher gene copies. Of the avian-associated AV4143 and GFD assays, the AV4143 was modified here with the accuracy of 88% which proved its potential in identifying faecal pollution from avian. For all poultry-associated markers tested, the best performance characteristics were shown in marker Ckmito (accuracy of 97%), followed by ND5-CD (accuracy of 88%), and finally LA35 and Chicken-Bac (accuracy of <= 77%). A combination of mitochondrial DNA (mtDNA) markers Ckmito and ND5-CD is recommended to differentiate the contamination caused by poultry. LA35 was more sensitive in poultry litter (100%) than in poultry faeces (21%). Our findings regarded BacUni, AV4143, and a combination of Ckmito and ND5-CD as the best performing universal, avian, and poultry assays, respectively. Suitable molecular markers could assist with efforts to determine the faecal pollution sources in environmental monitoring.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44189]
专题	生态环境研究中心_城市与区域生态国家重点实验室
推荐引用方式 GB/T 7714	Liang, Hongxia,Yu, Zhisheng,Ndayisenga, Fabrice,et al. A combination of mitochondrial DNA markers Ckmito and ND5-CD is recommended as the most reliable indicator for microbial source tracking to identify faecal pollution from poultry in China[J]. ECOLOGICAL INDICATORS,2020,115:1-10.
APA	Liang, Hongxia.,Yu, Zhisheng.,Ndayisenga, Fabrice.,Liu, Ruyin.,Zhang, Yiming.,...&Wu, Gang.(2020).A combination of mitochondrial DNA markers Ckmito and ND5-CD is recommended as the most reliable indicator for microbial source tracking to identify faecal pollution from poultry in China.ECOLOGICAL INDICATORS,115,1-10.
MLA	Liang, Hongxia,et al."A combination of mitochondrial DNA markers Ckmito and ND5-CD is recommended as the most reliable indicator for microbial source tracking to identify faecal pollution from poultry in China".ECOLOGICAL INDICATORS 115(2020):1-10.