Unexpected reversible and controllable nuclear uptake and efflux of the DNA light-switching'' Ru(II)-polypyridyl complex in living cells via ion-pairing with chlorophenolate counter-anions

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Unexpected reversible and controllable nuclear uptake and efflux of the DNA light-switching'' Ru(II)-polypyridyl complex in living cells via ion-pairing with chlorophenolate counter-anions
	Huang, Rong; Zhu, Jian-Qiang; Tang, Miao; Huang, Chun-Hua; Zhang, Zhi-Hui; Sheng, Zhi-Guo; Liu, Sijin; Zhu, Ben-Zhan
刊名	JOURNAL OF MATERIALS CHEMISTRY B
	2020-12-07
卷号	8 期号:45 页码:10327-10336
ISSN号	2050-750X
英文摘要	An in-depth understanding of the mechanisms of cellular uptake and efflux would facilitate the design of metal complexes with not only better functionality and targeted theranostic efficiency, but also with controlled toxicity. Here we find, unexpectedly, that the DNA light-switching'' Ru(II)-polypyridyl complex [Ru(phen)(2)(dppz)](2+) already delivered to the nucleus via ion-pairing with chlorophenolate counter-anions can gradually efflux to the cytoplasm when the cells were washed and incubated with fresh culture-medium. Interestingly, [Ru(phen)(2)(dppz)](2+) effluxed to the cytoplasm can be redirected back to the nucleus when the chlorophenolate counter-anions were added again. The efflux of nuclear [Ru(phen)(2)(dppz)](2+) was found to be mediated mainly via ATP-binding cassette (ABC) transporter proteins. Analogous reversible, but enantioselective nuclear uptake and efflux were observed with the two pure chiral forms of [Ru(phen)(2)(dppz)]Cl-2. This represents the first report of reversible and controllable nuclear uptake and efflux of a DNA light-switching'' Ru(II)-complex in living-cells via ion-pairing, which should provide novel insights for future research on using ion-pairing as an effective approach to control the cellular uptake and redistribution of other potential theranostic metal complexes.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44862]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Huang, Rong,Zhu, Jian-Qiang,Tang, Miao,et al. Unexpected reversible and controllable nuclear uptake and efflux of the DNA light-switching'' Ru(II)-polypyridyl complex in living cells via ion-pairing with chlorophenolate counter-anions[J]. JOURNAL OF MATERIALS CHEMISTRY B,2020,8(45):10327-10336.
APA	Huang, Rong.,Zhu, Jian-Qiang.,Tang, Miao.,Huang, Chun-Hua.,Zhang, Zhi-Hui.,...&Zhu, Ben-Zhan.(2020).Unexpected reversible and controllable nuclear uptake and efflux of the DNA light-switching'' Ru(II)-polypyridyl complex in living cells via ion-pairing with chlorophenolate counter-anions.JOURNAL OF MATERIALS CHEMISTRY B,8(45),10327-10336.
MLA	Huang, Rong,et al."Unexpected reversible and controllable nuclear uptake and efflux of the DNA light-switching'' Ru(II)-polypyridyl complex in living cells via ion-pairing with chlorophenolate counter-anions".JOURNAL OF MATERIALS CHEMISTRY B 8.45(2020):10327-10336.