Silver nanoparticles induce size-dependent and particle-specific neurotoxicity to primary cultures of rat cerebral cortical neurons

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Silver nanoparticles induce size-dependent and particle-specific neurotoxicity to primary cultures of rat cerebral cortical neurons
	Zhang, Bingjie; Liu, Na; Liu, Qian S.; Zhang, Jianqing; Zhou, Qunfang; Jiang, Guibin
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2020-07-15
卷号	198 页码:1-11
关键词	Silver nanoparticles Size-dependent effect Particle-specific effect Neurotoxicity Primary cultures of cerebral cortical neurons
ISSN号	0147-6513
英文摘要	Silver nanoparticles (AgNPs) are widely applied in many fields because of their excellent antibacterial activities. Toxicological studies have showed that AgNPs can cross the blood-brain barrier and exhibit high retention in the brain. Therefore, the potential neurotoxicity of AgNPs is raising serious concerns. This study investigated the neurotoxicological effects of AgNPs with two different sizes (20 nm and 70 nm, AgNPs-20 and AgNPs-70) using primary cultures of rat cerebral cortical neurons in mature and developing stages. The contribution of silver ion release was investigated by testing the effects of ionic silver in parallel. The results showed that both AgNPs-20 and AgNPs-70 significantly decreased neuronal cell viability, and AgNPs-20 had stronger toxicity compared with AgNPs-70. AgNP applications caused the granulated skeleton structure of the mature neurons with some broken synapses after a 24-h exposure, and inhibited neuronal growth during a 7-day exposure. Intracellular silver accumulation at non-cytotoxic exposure levels inhibited dopamine efflux, which was particle-specific and free of released silver ions. The findings herein can aid in guiding the proper applications of AgNPs in different areas, especially in medical use.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44808]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhang, Bingjie,Liu, Na,Liu, Qian S.,et al. Silver nanoparticles induce size-dependent and particle-specific neurotoxicity to primary cultures of rat cerebral cortical neurons[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,198:1-11.
APA	Zhang, Bingjie,Liu, Na,Liu, Qian S.,Zhang, Jianqing,Zhou, Qunfang,&Jiang, Guibin.(2020).Silver nanoparticles induce size-dependent and particle-specific neurotoxicity to primary cultures of rat cerebral cortical neurons.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,198,1-11.
MLA	Zhang, Bingjie,et al."Silver nanoparticles induce size-dependent and particle-specific neurotoxicity to primary cultures of rat cerebral cortical neurons".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 198(2020):1-11.