Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs
	Li, Heng; Liu, Shiqi; Hu, Yumeng; Zhao, Bin; Sun, Yeqing; Xu, Dan
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2020-04-01
卷号	192 页码:1-9
关键词	Endosulfan Cell migration PTP4A3 Signaling pathway Endothelial cells
ISSN号	0147-6513
英文摘要	Endosulfan is a persistent organic pollutant and can cause endothelial dysfunction, closely related to cardiovascular diseases. Endothelial cell migration plays a critical role in atherosclerosis and angiogenesis. This study was aimed to investigate the effect of environmentally relevant doses of endosulfan and underlying molecular mechanism on endothelial cell migration. Human umbilical vein endothelial cells (HUVECs) were treated with DMSO (control) or endosulfan (0.1, 1, 10 and 20 mu M) in the presence or absence of inhibitors. Wound healing and Transwell assay were employed to explore the effect of endosulfan on endothelial cell migration. The expression of genes or proteins was assayed by real-time PCR or immunoblotting. The results showed that endosulfan at relative low concentration (0.1, 1, 10 and 20 mu M) increased cell migration ability horizontally and vertically at 12 h after exposure. In line with this cellular effect, Protein-tyrosine Phosphatase 4A3 (PTP4A3) expression was significantly increased in endosulfan-exposed endothelial cells. Specific inhibitor of PTP4A3 significantly inhibited 20 mu M endosulfan-induced cell migration, the expression and phosphorylation of Src and phosphorylation of focal adhesion kinase (FAK). Exposure to endosulfan resulted in activation of various signaling pathways including phosphoinositide 3-kinase (PI3K)/AKT, mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-kappa B), which were suppressed by PTP4A3 inhibitor or specific inhibitor for each signaling pathway. Exposure to endosulfan significantly reduced nitric oxide production and caused oxidative stress in HUVECs. These findings suggest that endosulfan promoted cell migration through PTP4A3-mediated various signaling pathways in endothelial cells.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44672]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Li, Heng,Liu, Shiqi,Hu, Yumeng,et al. Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,192:1-9.
APA	Li, Heng,Liu, Shiqi,Hu, Yumeng,Zhao, Bin,Sun, Yeqing,&Xu, Dan.(2020).Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,192,1-9.
MLA	Li, Heng,et al."Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 192(2020):1-9.