Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on spontaneous movement of human neuroblastoma cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on spontaneous movement of human neuroblastoma cells
	Luo, Yali; Xu, Tuan; Xie, Heidi Qunhui; Guo, Zhiling; Zhang, Wanglong; Chen, Yangsheng; Sha, Rui; Liu, Yiyun; Ma, Yongchao; Xu, Li
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2020-05-01
卷号	715 页码:1-16
关键词	Dioxin Neuroblastoma Spontaneous movement Aryl hydrocarbon receptor Cell adhesion
ISSN号	0048-9697
英文摘要	Aryl hydrocarbon receptor (AhR) plays important roles in the interferences of dioxin exposure with the occurrence and development of tumors. Neuroblastoma is a kind of malignant tumor with high mortality and its occurrence is getting higher in dioxin exposed populations. However, there is still a lack of direct evidence of influences of dioxin on neuroblastoma cell migration. SK-N-SH is a human neuroblastoma cell line which has been used to reveal 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced dysregulation of certain promigratory gene. Thus, in this study, we employed SK-N-SH cells to investigate the effects of TCDD on the spontaneous movement of neuroblastoma cells, which is a short-range cell migratory behavior related to done formation and tumor metastasis in vitro. Using unlabeled live cell imaging and high content analysis, we characterized the spontaneous movement under a full-nutrient condition in SK-N-SH cells. We found that the spontaneous movement of SK-N-SH cells was inhibited after 36- or 48-h treatment with TCDD at relative low concentrations (10(-10) or 2 x 10(-10) M). The TCDD-treated cells were unable to move as freely as that of control cells, resulting in less diffusive trajectories and a decreased displacement of the movement. In line with this cellular effect, the expression of pro-adhesive genes was significantly induced in time- and concentration-dependent manners after TCDD treatment. In addition, with the presence of AhR antagonist, CH223191, the effects of TCDD on the gene expression and the spontaneous cell movement were effectively reversed. Thus, we proposed that AhR-mediated upregulation of pro-adhesive genes might be involved in the inhibitory effects of dioxin on the spontaneous movement of neuroblastoma cells. To our knowledge, this is the first piece of direct evidence about the influence of dioxin on neuroblastoma cell motility. (C) 2020 Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44664]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Luo, Yali,Xu, Tuan,Xie, Heidi Qunhui,et al. Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on spontaneous movement of human neuroblastoma cells[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,715:1-16.
APA	Luo, Yali.,Xu, Tuan.,Xie, Heidi Qunhui.,Guo, Zhiling.,Zhang, Wanglong.,...&Zhao, Bin.(2020).Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on spontaneous movement of human neuroblastoma cells.SCIENCE OF THE TOTAL ENVIRONMENT,715,1-16.
MLA	Luo, Yali,et al."Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on spontaneous movement of human neuroblastoma cells".SCIENCE OF THE TOTAL ENVIRONMENT 715(2020):1-16.