SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza | |
Li, Wenrui1,2; Xing, Bingcong1,2; Mao, Renjun3; Bai,Zhenqing3; Yang, Dongfeng4; Xu, Jiayi3; Liang, Zongsuo4 | |
刊名 | Industrial Crops & Products |
2020 | |
期号 | 14页码:112004 |
关键词 | Salvia Miltiorrhiza Tanshinones Gras Ga Signaling Regulation |
DOI | https://doi.org/10.1016/j.indcrop.2019.112004 |
英文摘要 | Salvia miltiorrhiza Bunge is the origin plant of traditional Chinese medicine, Danshen. Demand of high-quality S. miltiorrhiza is increasing because of its excellent pharmaceutical value. Tanshinones and salvianolic acids (SAs) are the main active ingredients of S. miltiorrhiza. GA was reported to induce tanshinones and SAs accumulation, however, its mechanism was unclear. Here, we got a GA responsive protein, SmGRAS3. SmGRAS3 expressed highest in the periderm where tanshinones produced and accumulated. Results of SmGRAS3 over and antisense expression indicated that SmGRAS3 is a positive regulator of tanshinones biosynthesis in S. miltiorrhiza hairy roots. But overexpression of SmGRAS3 depressed SAs and GA biosynthesis as well as hairy roots growth, and application of GA could partially recover these depressions. Pathway enzyme genes expressions of tanshinones, SAs and GA biosynthesis were comprehensively changed in SmGRAS3 transgenic hairy roots. SmGRAS3 acted as a transcription factor that located in nucleus and has the transcription activation activity. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) showed that SmGRAS3 directly bound to GARE-motif in promoter of KSL1 and activated KSL1 transcription. Our study suggested that the SmGRAS3 may be a good potential target for further metabolic engineering of bioactive components biosynthesis in S. miltiorrhiza. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.iswc.ac.cn/handle/361005/9189] |
专题 | 水保所2018届毕业生论文 |
通讯作者 | Liang, Zongsuo |
作者单位 | 1.Institute of Soil and Water Conservation, Chinese Academy of Sciences & Ministry of Water Resources, Yangling, 712100, China 2.University of the Chinese Academy of Sciences, Beijing, 100049, China 3.College of Life Sciences, Northwest A&F University, Yangling, 712100, China 4.College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China |
推荐引用方式 GB/T 7714 | Li, Wenrui,Xing, Bingcong,Mao, Renjun,et al. SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza[J]. Industrial Crops & Products,2020(14):112004. |
APA | Li, Wenrui.,Xing, Bingcong.,Mao, Renjun.,Bai,Zhenqing.,Yang, Dongfeng.,...&Liang, Zongsuo.(2020).SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza.Industrial Crops & Products(14),112004. |
MLA | Li, Wenrui,et al."SmGRAS3 negatively responds to GA signaling while promotes tanshinones biosynthesis in Salvia miltiorrhiza".Industrial Crops & Products .14(2020):112004. |
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