A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses | |
Wang, Xu2,3,4,5; Bian, Yangyang1; Cheng, Kai1; Zou, Hanfa1; Sun, Samuel Sai-Ming4,5; He, Jun-Xian4,5 | |
刊名 | journal of proteome research
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2012-04-01 | |
卷号 | 11期号:4页码:2301-2315 |
关键词 | proteomics phosphoproteomics nitrogen (N) nitrate N signaling N use efficiency Arabidopsis thaliana |
ISSN号 | 1535-3893 |
通讯作者 | 邹汉法 ; samuelsaimingsun ; junxianhe |
产权排序 | 4,2 |
英文摘要 | nitrogen (n) is an important nutrient and signal for plant growth and development. however, to date, our knowledge of how plants sense and transduce the n signals is very limited. to better understand the molecular mechanisms of plant n responses, we took two-dimensional gel-based proteomic and phosphoproteomic approaches to profile the proteins with abundance and phosphorylation state changes during nitrate deprivation and recovery in the model plant arabidopsis thaliana. after 7-day-old seedlings were n-deprived for up to 48 h followed by 24 h recovery, a total of 170 and 38 proteins were identified with significant changes in abundance and phosphorylation state, respectively. bioinformatic analyses implicate these proteins in diverse cellular processes including n and protein metabolisms, photosynthesis, cytoskeleton, redox homeostasis, and signal transduction. functional studies of the selected nitrate-responsive proteins indicate that the proteasome regulatory subunit rpt5a and the cytoskeleton protein tubulin alpha-6 (tua6) play important roles in plant nit-rate responses by regulating plant n use efficiency (nue) and low nitrate-induced anthocyanin biosynthesis, respectively. in conclusion, our study provides novel insights into plant responses to nitrate at the proteome level, which are expected to be highly useful for dissecting the n response pathways in higher plants and for improving plant nue. |
学科主题 | 物理化学 |
类目[WOS] | biochemical research methods |
研究领域[WOS] | biochemistry & molecular biology |
关键词[WOS] | gene-expression ; amino-acids ; root ; thaliana ; assimilation ; metabolism ; biosynthesis ; limitation ; database ; growth |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000302388100023 |
公开日期 | 2013-10-11 |
内容类型 | 期刊论文 |
源URL | [http://159.226.238.44/handle/321008/118307] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China 3.Tsinghua Univ, Div Life Sci, Grad Sch Shenzhen, Shenzhen 518055, Guangdong, Peoples R China 4.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong, Peoples R China 5.Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Xu,Bian, Yangyang,Cheng, Kai,et al. A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses[J]. journal of proteome research,2012,11(4):2301-2315. |
APA | Wang, Xu,Bian, Yangyang,Cheng, Kai,Zou, Hanfa,Sun, Samuel Sai-Ming,&He, Jun-Xian.(2012).A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses.journal of proteome research,11(4),2301-2315. |
MLA | Wang, Xu,et al."A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses".journal of proteome research 11.4(2012):2301-2315. |
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