A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses

	A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses
	Wang, Xu 2,3,4,5; Bian, Yangyang 1; Cheng, Kai 1; Zou, Hanfa 1; Sun, Samuel Sai-Ming 4,5; He, Jun-Xian 4,5
刊名	journal of proteome research
	2012-04-01
卷号	11 期号:4 页码:2301-2315
关键词	proteomics phosphoproteomics nitrogen (N) nitrate N signaling N use efficiency Arabidopsis thaliana
ISSN号	1535-3893
通讯作者	邹汉法 ; samuelsaimingsun ; junxianhe
产权排序	4,2
英文摘要	nitrogen (n) is an important nutrient and signal for plant growth and development. however, to date, our knowledge of how plants sense and transduce the n signals is very limited. to better understand the molecular mechanisms of plant n responses, we took two-dimensional gel-based proteomic and phosphoproteomic approaches to profile the proteins with abundance and phosphorylation state changes during nitrate deprivation and recovery in the model plant arabidopsis thaliana. after 7-day-old seedlings were n-deprived for up to 48 h followed by 24 h recovery, a total of 170 and 38 proteins were identified with significant changes in abundance and phosphorylation state, respectively. bioinformatic analyses implicate these proteins in diverse cellular processes including n and protein metabolisms, photosynthesis, cytoskeleton, redox homeostasis, and signal transduction. functional studies of the selected nitrate-responsive proteins indicate that the proteasome regulatory subunit rpt5a and the cytoskeleton protein tubulin alpha-6 (tua6) play important roles in plant nit-rate responses by regulating plant n use efficiency (nue) and low nitrate-induced anthocyanin biosynthesis, respectively. in conclusion, our study provides novel insights into plant responses to nitrate at the proteome level, which are expected to be highly useful for dissecting the n response pathways in higher plants and for improving plant nue.
学科主题	物理化学
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	gene-expression ; amino-acids ; root ; thaliana ; assimilation ; metabolism ; biosynthesis ; limitation ; database ; growth
收录类别	SCI
语种	英语
WOS记录号	WOS:000302388100023
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118307]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China 3.Tsinghua Univ, Div Life Sci, Grad Sch Shenzhen, Shenzhen 518055, Guangdong, Peoples R China 4.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong, Peoples R China 5.Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xu,Bian, Yangyang,Cheng, Kai,et al. A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses[J]. journal of proteome research,2012,11(4):2301-2315.
APA	Wang, Xu,Bian, Yangyang,Cheng, Kai,Zou, Hanfa,Sun, Samuel Sai-Ming,&He, Jun-Xian.(2012).A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses.journal of proteome research,11(4),2301-2315.
MLA	Wang, Xu,et al."A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses".journal of proteome research 11.4(2012):2301-2315.