Development of a combined chemical and enzymatic approach for the mass spectrometric identification and quantification of aberrant N-glycosylation

	Development of a combined chemical and enzymatic approach for the mass spectrometric identification and quantification of aberrant N-glycosylation
	Chen, Rui 1; Wang, Fangjun 1; Tan, Yexiong 2; Sun, Zhen 1; Song, Chunxia 1; Ye, Mingliang 1; Wang, Hongyang 2; Zou, Hanfa 1
刊名	journal of proteomics
	2012-02-16
卷号	75 期号:5 页码:1666-1674
关键词	Aberrant glycosylation Mass spectrometry Quantitative proteomics
ISSN号	1874-3919
通讯作者	邹汉法
产权排序	1,1
英文摘要	direct mass spectrometric analysis of aberrant protein glycosylation is a challenge to the current analytical techniques. except lectin affinity chromatography, no other glycosylation enrichment techniques are available for analysis of aberrant glycosylation. in this study, we developed a combined chemical and enzymatic strategy as an alternative for the mass spectrometric analysis of aberrant glycosylation. sialylated glycopeptides were enriched with reverse glycoblotting, cleaved by endoglycosidase f3 and analyzed by mass spectrometry with both neutral loss triggered ms3 in collision induced dissociation (cid) and electron transfer dissociation (etd). interestingly, a great part of resulted glycopeptides were found with fucose attached to the n-acetylglucosamine (n-glcnac), which indicated that the aberrant glycosylaton that is carrying both terminal sialylation and core fucosylation was identified. totally, 69 aberrant n-glycosylation sites were identified in sera samples from hepatocellular carcinoma (hcc) patients. following the identification, quantification of the level of this aberrant glycosylation was also carried out using stable isotope dimethyl labeling and pooled sera sample from liver cirrhosis and hcc was compared. six glycosylation sites demonstrated elevated level of aberrancy, which demonstrated that our developed strategy was effective in both qualitative and quantitative studies of aberrant glycosylation. (c) 2011 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	electron-transfer dissociation ; hepatocellular-carcinoma ; targeted glycoproteomics ; capture dissociation ; serum glycoproteins ; peptide ; chromatography ; fragmentation ; glycopeptides ; fucosylation
收录类别	SCI
语种	英语
WOS记录号	WOS:000300924700016
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118109]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Second Mil Med Univ, Eastern Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China
推荐引用方式 GB/T 7714	Chen, Rui,Wang, Fangjun,Tan, Yexiong,et al. Development of a combined chemical and enzymatic approach for the mass spectrometric identification and quantification of aberrant N-glycosylation[J]. journal of proteomics,2012,75(5):1666-1674.
APA	Chen, Rui.,Wang, Fangjun.,Tan, Yexiong.,Sun, Zhen.,Song, Chunxia.,...&Zou, Hanfa.(2012).Development of a combined chemical and enzymatic approach for the mass spectrometric identification and quantification of aberrant N-glycosylation.journal of proteomics,75(5),1666-1674.
MLA	Chen, Rui,et al."Development of a combined chemical and enzymatic approach for the mass spectrometric identification and quantification of aberrant N-glycosylation".journal of proteomics 75.5(2012):1666-1674.