Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes

	Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes
	Wang, Fangjun; Zhu, Jun; Hu, Lianghai; Qin, Hongqiang; Ye, Mingliang; Zou, Hanfa
刊名	protein & cell
	2012-09-01
卷号	3 期号:9 页码:669-674
关键词	human serum endogenous peptide N and C termini disease diagnosis
ISSN号	1674-800x
通讯作者	邹汉法
产权排序	1,1
英文摘要	the human serum proteome is closely associated with the state of the body. endogenous peptides derived from proteolytic enzymes cleaving on serum proteins are widely studied due to their potential application in disease-specific marker discovery. however, the reproducibility of peptidome analysis of endogenous peptides is significantly influenced by the proteolytic enzymes within body fluids, thereby limiting the clinical use of the endogenous peptides. we comprehensively investigated the n and c terminus of endogenous peptides using peptidomics. the cleavage site patterns of the n and c terminus and adjacent sites from all the identified endogenous peptides were highly conserved under different sample preparation conditions, including long-term incubation at 37 degrees c and pretreatment with repeated freeze-thaw cycles. furthermore, a distinguishable cleavage site pattern was obtained when a different disease serum was analyzed. the conserved cleavage site pattern derived from proteolytic enzymes holds potential in highly specific disease diagnosis.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	cell biology
研究领域[WOS]	cell biology
关键词[WOS]	mass-spectrometry ; proteome ; plasma ; quantification ; peptidomics
收录类别	SCI
语种	英语
WOS记录号	WOS:000310532800004
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118013]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Fangjun,Zhu, Jun,Hu, Lianghai,et al. Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes[J]. protein & cell,2012,3(9):669-674.
APA	Wang, Fangjun,Zhu, Jun,Hu, Lianghai,Qin, Hongqiang,Ye, Mingliang,&Zou, Hanfa.(2012).Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes.protein & cell,3(9),669-674.
MLA	Wang, Fangjun,et al."Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes".protein & cell 3.9(2012):669-674.