Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline

doi:10.3390/cryst10090798

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline
	Liang, M ; Wang, ZJ ; Wu, H ; Yu, L ; Sun, B ; Zhou, H ; Yu, F ; Wang, QS ; He, JH
刊名	CRYSTALS
	2020
卷号	10 期号:9 页码:-
关键词	X-RAY-DIFFRACTION PROTEIN CRYSTALLIZATION CRYSTALLOGRAPHY CHIP SOFTWARE PLATFORM DROPLET
ISSN号	2073-4352
DOI	10.3390/cryst10090798
文献子类	期刊论文
英文摘要	An efficient data collection method is important for microcrystals, because microcrystals are sensitive to radiation damage. Moreover, microcrystals are difficult to harvest and locate owing to refraction effects from the surface of the liquid drop or optically invisible, owing to their small size. Collecting X-ray diffraction data directly from the crystallization devices to completely eliminate the crystal harvesting step is of particular interest. To address these needs, novel microplates combining crystal growth and data collection have been designed for efficient in situ data collection and fully tested at Shanghai Synchrotron Radiation Facility (SSRF) crystallography beamlines. The design of the novel microplates fully adapts the advantage of in situ technology. Thin Kapton membranes were selected to seal the microplate for crystal growth, the crystallization plates can support hanging drop and setting drop vapor diffusion crystallization experiments. Then, the microplate was fixed on a magnetic base and mounted on the goniometer head for in situ data collection. Automatic grid scanning was applied for crystal location with a Blu-Ice data collection system and then in situ data collection was performed. The microcrystals of lysozyme were selected as the testing samples for diffraction data collection using the novel microplates. The results show that this method can achieve comparable data quality to that of the traditional method using the nylon loop. In addition, our method can efficiently and diversely perform data acquisition experiments, and be especially suitable for solving structures of multiple crystals at room temperature or cryogenic temperature.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/33005]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Wuhan Univ, Inst Adv Studies, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China
推荐引用方式 GB/T 7714	Liang, M,Wang, ZJ,Wu, H,et al. Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline[J]. CRYSTALS,2020,10(9):-.
APA	Liang, M.,Wang, ZJ.,Wu, H.,Yu, L.,Sun, B.,...&He, JH.(2020).Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline.CRYSTALS,10(9),-.
MLA	Liang, M,et al."Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline".CRYSTALS 10.9(2020):-.