A efficient method to identify cardioprotective components of Astragali Radix using a combination of molecularly imprinted polymers-based knockout extract and activity evaluation

doi:10.1016/j.chroma.2018.09.027

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 国家天然药物工程技术研究中心 > 天然产物研究

	A efficient method to identify cardioprotective components of Astragali Radix using a combination of molecularly imprinted polymers-based knockout extract and activity evaluation
	Xie, Jing 2,3; Xiong, Jing 3; Ding, Li-Sheng 1; Chen, Li 2; Zhou, Hua 2; Liu, Liang 2; Zhang, Zhi-Feng 2; Hu, Xue-Mei 3; Luo, Pei 2; Qing, Lin-Sen 1,3
刊名	JOURNAL OF CHROMATOGRAPHY A
	2018
卷号	1576 期号:2018 页码:10-18
关键词	Astragali Radix Calycosin molecularly imprinted polymer Chemical compounds' knockout Hypoxia/reoxygenation model Holistic evaluation
ISSN号	0021-9673
DOI	10.1016/j.chroma.2018.09.027
产权排序	3
文献子类	Article
英文摘要	Although herb medicines have become the major source for new drug discovery, many of them are largely under-explored due to the purity-activity relationship. Efficient identification of bioactive compounds in conventional stepwise separation and isolation has not yet been elucidated. Therefore, we proposed a new separation strategy for holism understanding of herb pharmacology using molecularly imprinted polymers (MIPs). Astragali Radix (AR), important in traditional Chinese medicine, was chosen in this study for multicomponent knockout followed by bioactivity evaluation. We prepared calycosin molecularly imprinted polymers (calycosin-MIPs) which could selectively recognize flavonoid aglycons in AR. The molecular selectivity of calycosin-MIPs as a critical parameter was evaluated using the template and other high content compounds in AR. Based on it, using the calycosin-MIPs material via solid-phase extraction procedures was applied for the knockout of flavonoid aglycons in AR. Finally, hypoxia/reoxygenation model in H9c2 cells was used to evaluate the activity of the AR extract before and after knockout. The results showed that calycosin-MIPs as recognition materials for flavonoid aglycons in AR are applied in one-step separation with high selectivity and tunability. The subextract in the absence of flavonoid aglycons has been demonstrated to clarify the cardio-protective components of AR. In conclusion, this proof-of-principle study is the first one showing that molecular imprinting technology coupled with a bioassay can be used to explore the bioactive variability from the perspective of multicomponent separation of herbal medicine. (C) 2018 Elsevier B.V. All rights reserved.
学科主题	Chemistry ; Analysis
URL标识	查看原文
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; HERBAL MEDICINES ; MASS-SPECTROMETRY ; SOLID-PHASE ; STRATEGY ; CONSTITUENTS ; MARKERS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000450383300002
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30629]
专题	国家天然药物工程技术研究中心_天然产物研究
作者单位	1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China 2.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau, Peoples R China; 3.Chengdu Med Coll, Sch Pharm, Chengdu 610500, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Xie, Jing,Xiong, Jing,Ding, Li-Sheng,et al. A efficient method to identify cardioprotective components of Astragali Radix using a combination of molecularly imprinted polymers-based knockout extract and activity evaluation[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1576(2018):10-18.
APA	Xie, Jing.,Xiong, Jing.,Ding, Li-Sheng.,Chen, Li.,Zhou, Hua.,...&Qing, Lin-Sen.(2018).A efficient method to identify cardioprotective components of Astragali Radix using a combination of molecularly imprinted polymers-based knockout extract and activity evaluation.JOURNAL OF CHROMATOGRAPHY A,1576(2018),10-18.
MLA	Xie, Jing,et al."A efficient method to identify cardioprotective components of Astragali Radix using a combination of molecularly imprinted polymers-based knockout extract and activity evaluation".JOURNAL OF CHROMATOGRAPHY A 1576.2018(2018):10-18.