Comparative genomic investigation of high-elevation adaptation in ectothermic snakes

doi:10.1073/pnas.1805348115

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 生物多样性与生态系统服务领域 > 中国科学院山地生态恢复与生物资源利用重点实验室

	Comparative genomic investigation of high-elevation adaptation in ectothermic snakes
	Li, Jia-Tang 1,2; Gao, Yue-Dong 8; Xie, Liang 7; Deng, Cao 6; Shi, Peng 1,8; Guan, Meng-Long 2; Huang, Song 5; Ren, Jin-Long 2,4; Wu, Dong-Dong 1,8; Ding, Li 2
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2018
卷号	115 期号:33 页码:8406-8411
关键词	snakes de novo genome comparative genomics positive selection high-elevation adaptation
ISSN号	0027-8424
DOI	10.1073/pnas.1805348115
产权排序	1
文献子类	Article
英文摘要	Several previous genomic studies have focused on adaptation to high elevations, but these investigations have been largely limited to endotherms. Snakes of the genus Thermophis are endemic to the Tibetan plateau and therefore present an opportunity to study high-elevation adaptations in ectotherms. Here, we report the de novo assembly of the genome of a Tibetan hot-spring snake (Thermophis baileyi) and then compare its genome to the genomes of the other two species of Thermophis, as well as to the genomes of two related species of snakes that occur at lower elevations. We identify 308 putative genes that appear to be under positive selection in Thermophis. We also identified genes with shared amino acid replacements in the high-elevation hot-spring snakes compared with snakes and lizards that live at low elevations, including the genes for proteins involved in DNA damage repair (FEN1) and response to hypoxia (EPAS1). Functional assays of the FEN1 alleles reveal that the Thermophis allele is more stable under UV radiation than is the ancestral allele found in low-elevation lizards and snakes. Functional assays of EPAS1 alleles suggest that the Thermophis protein has lower transactivation activity than the low-elevation forms. Our analysis identifies some convergent genetic mechanisms in high-elevation adaptation between endotherms (based on studies of mammals) and ectotherms (based on our studies of Thermophis).
学科主题	Multidisciplinary
URL标识	查看原文
WOS关键词	RNA-BINDING PROTEIN ; HIGH-ALTITUDE ADAPTATION ; REPAIR DEFICIENCY ; P53 FAMILY ; MUTATIONS ; EVOLUTION ; SEQUENCE ; REVEALS ; STABILITY ; INSIGHTS
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000441638200063
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30611]
专题	生物多样性与生态系统服务领域_中国科学院山地生态恢复与生物资源利用重点实验室
作者单位	1.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China; 2.CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, 610040 Chengdu, China; 3.Department of Integrative Biology and Biodiversity Center, University of Texas at Austin, Austin, TX 78712; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 5.Huangshan Univ, Coll Life & Environm Sci, Huangshan 245041, Peoples R China; 6.DNA Stories Bioinformat Ctr, Dept Bioinformat, Chengdu 610041, Peoples R China; 7.Sichuan Univ, West China Univ Hosp 2, Minist Educ, Key Lab Birth Defects & Related Dis Women & Child, Chengdu 610041, Peoples R China; 8.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China; 9.Guizhou Acad Testing & Anal, Guiyang 550014, Guizhou, Peoples R China
推荐引用方式 GB/T 7714	Li, Jia-Tang,Gao, Yue-Dong,Xie, Liang,et al. Comparative genomic investigation of high-elevation adaptation in ectothermic snakes[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(33):8406-8411.
APA	Li, Jia-Tang.,Gao, Yue-Dong.,Xie, Liang.,Deng, Cao.,Shi, Peng.,...&Zhang, Ya-Ping.(2018).Comparative genomic investigation of high-elevation adaptation in ectothermic snakes.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(33),8406-8411.
MLA	Li, Jia-Tang,et al."Comparative genomic investigation of high-elevation adaptation in ectothermic snakes".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.33(2018):8406-8411.