Use of electrospray ionization ion-trap tandem mass spectrometry and principal component analysis to directly distinguish monosaccharides

doi:10.1002/rcm.6219

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 生物多样性与生态系统服务领域 > 中国科学院山地生态恢复与生物资源利用重点实验室

	Use of electrospray ionization ion-trap tandem mass spectrometry and principal component analysis to directly distinguish monosaccharides
	Xia, Bing 3; Zhou, Yan 3; Liu, Xin 2; Xiao, Juan 3; Liu, Qing 3; Gu, Yucheng 1; Ding, Lisheng 3
刊名	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
	2012-06-15
卷号	26 期号:11 页码:1259-1264
ISSN号	0951-4198
DOI	10.1002/rcm.6219
产权排序	1
文献子类	Article
英文摘要	RATIONALE Carbohydrates are good source of drugs and play important roles in metabolism processes and cellular interactions in organisms. Distinguishing monosaccharide isomers in saccharide derivates is an important and elementary work in investigating saccharides. It is important to develop a fast, simple and direct method for this purpose, which is described in this study. METHODS Stock solutions of monosaccharide with a concentration of 400 mu M and sodium chloride at a concentration of 10 mu M were made in water/methanol (50:50, v/v). The samples were subjected to electrospray ionization ion-trap tandem mass spectrometry (ESI-MS) and the detected [2M?+?Na H2O]+ ions were further investigated by tandem mass spectrometry (MS/MS), followed by applying principal component analysis (PCA) on the obtained MS/MS data sets. RESULTS The MS/MS spectra of the [2M?+?Na H2O]+ ions at m/z 365 for hexoses and m/z 305 for pentoses yielded unambiguous fragment patterns, while rhamnose can be directly identified by its ESI-MS [M?+?Na]+ ion at m/z 187. PCA showed clustering of MS/MS data of identical monosaccharide samples obtained from different experiments. By using this method, the monosaccharide in daucosterol hydrolysate was successfully identified. CONCLUSIONS A new strategy was developed for differentiation of the monosaccharides using ESI-MS/MS and PCA. In MS/MS spectra, the [2M?+?Na H2O]+ ions yielded unambiguous distinction. PCA of the archived MS/MS data sets was applied to demonstrate the spatial resolution of the studied samples. This method presented a simple and reliable way for distinguishing monosaccharides by ESI-MS/MS. Copyright (C) 2012 John Wiley & Sons, Ltd.
学科主题	Spectroscopy/instrumentation/analytical Sciences
URL标识	查看原文
WOS关键词	CARBOHYDRATE-BASED VACCINES ; DISACCHARIDES ; DIFFERENTIATION ; DISTINCTION ; ISOMERS ; HEXOSES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000303597100001
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/29919]
专题	生物多样性与生态系统服务领域_中国科学院山地生态恢复与生物资源利用重点实验室
作者单位	1.Syngenta Jealotts Hill Int Res Ctr, Bracknell RG42 6EY, Berks, England 2.Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100026, Peoples R China; 3.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Mt Ecol Restorat & Bioresource Utilizat, Chengdu 610041, Peoples R China;
推荐引用方式 GB/T 7714	Xia, Bing,Zhou, Yan,Liu, Xin,et al. Use of electrospray ionization ion-trap tandem mass spectrometry and principal component analysis to directly distinguish monosaccharides[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2012,26(11):1259-1264.
APA	Xia, Bing.,Zhou, Yan.,Liu, Xin.,Xiao, Juan.,Liu, Qing.,...&Ding, Lisheng.(2012).Use of electrospray ionization ion-trap tandem mass spectrometry and principal component analysis to directly distinguish monosaccharides.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,26(11),1259-1264.
MLA	Xia, Bing,et al."Use of electrospray ionization ion-trap tandem mass spectrometry and principal component analysis to directly distinguish monosaccharides".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 26.11(2012):1259-1264.