Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.)

doi:10.1007/s00425-019-03278-0

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.)
	Cao, Pei 5; Liang, Xiaona 1,5; Zhao, Hong 1,5; Feng, Bo 4; Xu, Enjun 5; Wang, Liming 3; Hu, Yuxin 2,5
刊名	PLANTA
	2019
卷号	250 期号:6 页码:1967-1981
关键词	Grain length Grain weight KASP markers QTL Spike-related traits Wheat
ISSN号	0032-0935
DOI	10.1007/s00425-019-03278-0
产权排序	3
文献子类	Article
英文摘要	Main conclusionTotally, 48 loci responsible for six spike-related traits were identified in wheat, and a major locus QGl-4A for grain length was mapped and validated for marker-assisted selection in breeding.AbstractWheat yield is determined by the number of spikes, number of grains per spike (GN), and one-thousand kernel weight (TKW), among which GN and TKW are greatly related to the spike development and thus the spike-related traits, including spike length (SL), number of spikelet per spike (SN), grain length (GL) and grain width (GW). To identify the key loci governing the spike-related traits (SL, SN, GN, TKW, GL and GW), we conducted the quantitative trait loci (QTL) analysis combined with wheat 660K SNP chip and Kompetitive allele-specific PCR (KASP) assay, using the F-2 and F-2:3 populations derived from Luohan6 (LH6) with big spike and grain and Zhengmai366 with small spike and grain, and identified a total of 48 QTLs on 18 chromosomes. Moreover, a major stable QTL for GL on chromosome 4A, designated as QGl-4A, was mapped into a 0.37 cM interval between KASP markers Xib4A-10 and Xib4A-12, corresponding to 20 Mb physical region in the Chinese Spring genome. This QTL explained 17.30% and 5.12% of the phenotypic variation for GL in the F-2 and F-2:3 populations. Further association analysis of flanking markers Xib4A-10 and Xib4A-12 in 192 wheat varieties showed that these two markers could be used for marker-assisted selection in breeding. These results provide valuable information for map-based cloning of the target genes involved in the regulation of spike-related traits in common wheat.
学科主题	Plant Sciences
URL标识	查看原文
WOS关键词	STRIPE RUST RESISTANCE ; BREAD WHEAT ; GRAIN WEIGHT ; QTL ; YIELD ; GENE ; MARKERS ; ASSOCIATION ; ARRAY ; PCR
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000496035900013
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30882]
专题	环境治理与食品安全领域_农业生物技术研究
通讯作者	Hu, Yuxin
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China 3.Henan Sci & Technol Univ, Luoyang 471023, Peoples R China; 4.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China; 5.Chinese Acad Sci, Key Lab Plant Mol Physiol, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Beijing 100093, Peoples R China;
推荐引用方式 GB/T 7714	Cao, Pei,Liang, Xiaona,Zhao, Hong,et al. Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.)[J]. PLANTA,2019,250(6):1967-1981.
APA	Cao, Pei.,Liang, Xiaona.,Zhao, Hong.,Feng, Bo.,Xu, Enjun.,...&Hu, Yuxin.(2019).Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.).PLANTA,250(6),1967-1981.
MLA	Cao, Pei,et al."Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.)".PLANTA 250.6(2019):1967-1981.