Analysis of DNA Methylation of Wheat in Response to Low Nitrogen Stress Based on Mathylation-Sensitive Amplified Polymorphisms

doi:10.16373/j.cnki.ahr.180039

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	Analysis of DNA Methylation of Wheat in Response to Low Nitrogen Stress Based on Mathylation-Sensitive Amplified Polymorphisms
	Liao, Jinhua 1; Zhou, Min 2; Fan, Jin 1; Hu, Xia 1; Tang, Mei 1; Gong, Mingfu 1; Wu, Yu 2
刊名	INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
	2018-09-01
卷号	20 期号:12 页码:2897-2904
关键词	DNA methylation/demethylation Gene expression Low nitrogen MSAP Wheat grain
ISSN号	1560-8530
DOI	10.16373/j.cnki.ahr.180039
产权排序	2
文献子类	Article
英文摘要	Abiotic stress can induce considerable changes in the composition and quality of wheat grain and cause genomic DNA methylation. Here, methylation-sensitive amplified polymorphism (MSAP) was used to detect the effect of low nitrogen stress on the extent and patterns of DNA methylation in wheat grain at 5, 8, 11, 14, 16 and 21 days post-anthesis (DPA). First, our results showed that low nitrogen stress could reduce grain N content, sodium dodecylsulfate sedimentation (SDS) volume, the amounts of high-molecular-weight glutenin subunits (HMW-GS 2, 12 and 20) and total protein. Then, at 8, 11 and 21 DPA, the overall level of DNA methylation in low nitrogen group was higher than in control group with the maximum difference of 14.1% at 21 DPA. And, at 8 DPA, methylation / demethylation revealed the highest level under low nitrogen conditions, about 17.07% -30.99% of the CCGG sites. Thus, thirteen differentially displayed DNA fragments in MSAP profiles in samples at 8 DPA were further cloned for sequencing analysis. Homology analysis for these DNA fragments showed that most of them were involved in storage matter (protein, fatty acid), some engaged in stress signaling and physiological response regulation. Moreover, the relative expression levels revealed that the expression of a demethylated fragment was increased but the expressions of two other methylated fragments decreased. In conclusion, our results suggested that low nitrogen stress could decrease SDS volume, HMW-GS amount and total protein which might be associated with DNA methylation. (C) 2018 Friends Science Publishers
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WOS关键词	BREAD-MAKING QUALITY ; DROUGHT ; TEMPERATURE ; SUBUNITS ; GLUTENIN ; ENVIRONMENT ; PROTEIN ; WATER ; SALT
WOS研究方向	Agriculture ; Life Sciences & Biomedicine - Other Topics
语种	英语
出版者	FRIENDS SCIENCE PUBL
WOS记录号	WOS:000454160600036
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30356]
专题	环境治理与食品安全领域_农业生物技术研究
作者单位	1.Leshan Normal Univ, Coll Life Sci, Leshan 614000, Sichuan, Peoples R China; 2.Univ Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Liao, Jinhua,Zhou, Min,Fan, Jin,et al. Analysis of DNA Methylation of Wheat in Response to Low Nitrogen Stress Based on Mathylation-Sensitive Amplified Polymorphisms[J]. INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,2018,20(12):2897-2904.
APA	Liao, Jinhua.,Zhou, Min.,Fan, Jin.,Hu, Xia.,Tang, Mei.,...&Wu, Yu.(2018).Analysis of DNA Methylation of Wheat in Response to Low Nitrogen Stress Based on Mathylation-Sensitive Amplified Polymorphisms.INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,20(12),2897-2904.
MLA	Liao, Jinhua,et al."Analysis of DNA Methylation of Wheat in Response to Low Nitrogen Stress Based on Mathylation-Sensitive Amplified Polymorphisms".INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY 20.12(2018):2897-2904.