Methane oxidation coupled to denitrification under microaerobic and hypoxic conditions in leach bed bioreactors

doi:10.1016/j.scitotenv.2018.08.289

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 应用与环境微生物研究

	Methane oxidation coupled to denitrification under microaerobic and hypoxic conditions in leach bed bioreactors
	Cao, Qin 2; Liu, Xiaofeng 2; Ran, Yi 1,3; Li, Zhidong 2; Li, Dong 2
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2019
卷号	649 页码:1-11
关键词	Landfill Microaerobic methane oxidation Hypoxic methane oxidation Leachate denitrification Microbial community
ISSN号	0048-9697
DOI	10.1016/j.scitotenv.2018.08.289
产权排序	1
文献子类	Article
英文摘要	Managing nitrogen and carbon cycles in landfills is an environmental challenge. In this study, our purpose was to test two types of methane oxidation processes coupled to denitrification inside landfills: microaerobic and hypoxic methane oxidation coupled to denitrification (MAME-D and HYME-D). Leach bed bioreactors were designed and operated for >100 d with NO3- -N concentration ranging from 100 to 400 mg N/L During six runs of the leach bed bioreactor experiment, leach bed bioreactor 2 (MAME-D) reached 100% denitrification efficiency and the highest average specific denitrification rate of 20.36 mg N/(L.d) in run 5, while leach bed bioreactor 3 (HYME-D) achieved 75% denitrification efficiency and the highest average specific denitrification rate of 8.09 mg N/(L.d) in run 6. Subsequently, waste from leach bed bioreactors 1, 2, and 3 was inoculated into anaerobic bottles to run a batch experiment for 13 d. The total consumed methane, oxygen, and nitrate amounts in the microaerobic system with no methane and oxygen supplement were 2.33 2.38, and 2.04 mmol, respectively, which almost matched the theoretical equation of aerobic methane oxidation coupled to denitrification. In the hypoxic system, the total consumed methane and nitrate amounts were 023 and 0.41 mmol, respectively, the ratio of which dosely matched the HYME-D. In addition, via the diverse functional community analysis, methane oxidation in the microaerobic system was confirmed to be conducted by methanotrophs (i.e., Methylobacter and Methylomonas) using oxygen as an electron acceptor. Subsequently, the generated organic compounds could support denitrifiers (i.e., Methylophilaceae) to complete denitrification. In the hypoxic system, Methylomonas and Methylobacter utilized nitrate as a direct electron acceptor to oxidize methane. The two landfill processes characterized here will expand our understanding of the environmental role of methanotrophs and methylotrophs in both carbon and nitrogen cycles. (C) 2018 Elsevier B.V. All rights reserved.
学科主题	Environment/ecology
URL标识	查看原文
WOS关键词	EXTERNAL CARBON SOURCE ; NITRIFICATION ; METHANOTROPHS ; RECIRCULATION ; GROUNDWATER ; BIOGAS ; DONOR ; FIELD
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000446076500001
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30532]
专题	环境治理与食品安全领域_应用与环境微生物研究
通讯作者	Li, Dong
作者单位	1.Minist Agr, Key Lab Technol & Model Cycl Utilizat Agr Resourc, Chengdu, Sichuan, Peoples R China 2.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Environm Microbiol Key Lab Sichuan Prov, Chengdu 610091, Sichuan, Peoples R China; 3.Minist Agr, Blogas Inst, Chengdu 610041, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Cao, Qin,Liu, Xiaofeng,Ran, Yi,et al. Methane oxidation coupled to denitrification under microaerobic and hypoxic conditions in leach bed bioreactors[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2019,649:1-11.
APA	Cao, Qin,Liu, Xiaofeng,Ran, Yi,Li, Zhidong,&Li, Dong.(2019).Methane oxidation coupled to denitrification under microaerobic and hypoxic conditions in leach bed bioreactors.SCIENCE OF THE TOTAL ENVIRONMENT,649,1-11.
MLA	Cao, Qin,et al."Methane oxidation coupled to denitrification under microaerobic and hypoxic conditions in leach bed bioreactors".SCIENCE OF THE TOTAL ENVIRONMENT 649(2019):1-11.