Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor

doi:10.1007/s11103-018-0778-x

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 应用与环境微生物研究

	Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor
	Yang, Gui-Li 1,2,3; Fang, Yang 2,3; Xu, Ya-Liang 1,2,3; Tan, Li 2,3; Li, Qi 1,2,3; Liu, Yang 1,2,3; Lai, Fan 1,2,3; Jin, Yan-Ling 2,3; Du, An-Ping 2,3; He, Kai-Ze 2,3
刊名	PLANT MOLECULAR BIOLOGY
	2018-11-01
卷号	98 期号:2018 页码:319-331
关键词	Duckweed Lemna minor Frond transformation system Agrobacterium tumefaciens Plant bioreactor Stigmasterol
ISSN号	0167-4412
DOI	10.1007/s11103-018-0778-x
产权排序	1
文献子类	Article
英文摘要	The Lemnaceae, known as duckweed, the smallest flowering aquatic plant, shows promise as a plant bioreactor. For applying this potential plant bioreactor, establishing a stable and efficient genetic transformation system is necessary. The currently favored callus-based method for duckweed transformation is time consuming and genotype limited, as it requires callus culture and regeneration, which is inapplicable to many elite duckweed strains suitable for bioreactor exploitation. In this study, we attempted to establish a simple frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor, one of the most widespread duckweed species in the world. To evaluate the feasibility of the new transformation system, the gene CYP710A11 was overexpressed to improve the yield of stigmasterol, which has multiple medicinal purposes. Three L. minor strains, ZH0055, D0158 and M0165, were transformed by both a conventional callus transformation system (CTS) and the simple frond transformation system (FTS). GUS staining, PCR, quantitative PCR and stigmasterol content detection showed that FTS can produce stable transgenic lines as well as CTS. Moreover, compared to CTS, FTS can avoid the genotype constraints of callus induction, thus saving at least half of the required processing time (CTS took 8-9 months while FTS took approximately 3 months in this study). Therefore, this transformation system is feasible in producing stable transgenic lines for a wide range of L. minor genotypes.
学科主题	Plant Sciences
URL标识	查看原文
WOS关键词	DISCHARGE PARTICLE-ACCELERATION ; CALLUS INDUCTION ; WASTE-WATER ; BIOMASS PRODUCTION ; DUCKWEED ; EXPRESSION ; STIGMASTEROL ; PLANTS ; REGENERATION ; GENE
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000450658300003
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30121]
专题	环境治理与食品安全领域_应用与环境微生物研究
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China; 3.Environm Microbiol Key Lab Sichuan Prov, Chengdu 610041, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Yang, Gui-Li,Fang, Yang,Xu, Ya-Liang,et al. Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor[J]. PLANT MOLECULAR BIOLOGY,2018,98(2018):319-331.
APA	Yang, Gui-Li.,Fang, Yang.,Xu, Ya-Liang.,Tan, Li.,Li, Qi.,...&Zhao, Hai.(2018).Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor.PLANT MOLECULAR BIOLOGY,98(2018),319-331.
MLA	Yang, Gui-Li,et al."Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor".PLANT MOLECULAR BIOLOGY 98.2018(2018):319-331.