Biodegradation of phenanthrene at high concentrations by Acidovorax sp. JG5 and its functional genomic analysis

doi:10.1002/jctb.6867

	Biodegradation of phenanthrene at high concentrations by Acidovorax sp. JG5 and its functional genomic analysis
	Liao, Xiaoyong 1,3; Luo, Junpeng 1,3; Cassidy, Daniel P.2; Li, You 1,3; Tao, Huan 1,3; Zhao, Yishu 1,3
刊名	JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
	2021-08-12
页码	10
关键词	Acidovorax sp biodegradation metabolic pathways phenanthrene polycyclic aromatic hydrocarbons (PAH)
ISSN号	0268-2575
DOI	10.1002/jctb.6867
通讯作者	Liao, Xiaoyong(liaoxy@igsnrr.ac.cn)
英文摘要	BACKGROUND Phenanthrene (PHE) is a widespread, highly-toxic, and biodegradable polycyclic aromatic hydrocarbon (PAH) that can be found with high concentrations in multiple industrial sites. For the efficient biodegradation of PHE, especially for the high concentrations, a PHE-degrading Acidovorax strain was isolated from a soil contaminated for decades with PAH; its ability to degrade PHE was investigated and the pathways involved were identified. RESULTS A PHE-degrading strain was isolated and identified as Acidovorax sp. JG5. The bacterium completely degraded 200 mg center dot L-1 PHE in 24 h and it degraded over 90% of PHE in solutions ranging from 500 to 1500 mg center dot L-1 in 48 h. Key metabolites, such as 9,10-phenanthraquinone, 2-hydroxy-1-naphthoic acid, protocatechuic acid, phthalic acid, pyruvic acid, and salicylic acid, were detected during PHE biodegradation. Twelve genes related to PHE biodegradation (e.g., nahAa, pht5, ligA, and dmp cluster) were also revealed. Two PHE degradation pathways are proposed based on these metabolites and genes. CONCLUSION Acidovorax sp. JG5 exhibits a high tolerance to PHE and high rates of degradation, along with a tremendous potential for the bioremediation of heavy PAH contamination. (c) 2021 Society of Chemical Industry
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23010402] ; Fund Program of State Key Laboratory of NBC Protection for Civilian[sklnbc2018-17]
WOS关键词	POLYCYCLIC AROMATIC-HYDROCARBONS ; SP STRAIN PN/Y ; 2-HYDROXY-1-NAPHTHOIC ACID ; DEGRADATION ; SOIL ; TOXICITY ; BACTERIA ; PYRENE ; PAHS ; REMEDIATION
WOS研究方向	Biotechnology & Applied Microbiology ; Chemistry ; Engineering
语种	英语
出版者	WILEY
WOS记录号	WOS:000684181900001
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; Fund Program of State Key Laboratory of NBC Protection for Civilian
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/164635]
专题	中国科学院地理科学与资源研究所
通讯作者	Liao, Xiaoyong
作者单位	1.Chinese Acad Sci, Key Lab Land Surface Pattern & Simulat, Beijing, Peoples R China 2.Western Michigan Univ, Dept Geol & Environm Sci, Kalamazoo, MI 49008 USA 3.Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing Key Lab Environm Damage Assessment & Reme, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liao, Xiaoyong,Luo, Junpeng,Cassidy, Daniel P.,et al. Biodegradation of phenanthrene at high concentrations by Acidovorax sp. JG5 and its functional genomic analysis[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2021:10.
APA	Liao, Xiaoyong,Luo, Junpeng,Cassidy, Daniel P.,Li, You,Tao, Huan,&Zhao, Yishu.(2021).Biodegradation of phenanthrene at high concentrations by Acidovorax sp. JG5 and its functional genomic analysis.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,10.
MLA	Liao, Xiaoyong,et al."Biodegradation of phenanthrene at high concentrations by Acidovorax sp. JG5 and its functional genomic analysis".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2021):10.