A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification

doi:10.1021/acs.analchem.1c00796

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification
	Wang, Xiaokun 1; Shang, Hezhen 2; Ma, Cuiping 1; Chen, Lingxin 3,4,5
刊名	ANALYTICAL CHEMISTRY
	2021-06-22
卷号	93 期号:24 页码:8493-8500
ISSN号	0003-2700
DOI	10.1021/acs.analchem.1c00796
通讯作者	Ma, Cuiping(mcp169@163.com) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	Exosomes are emerging as one of the most promising biomarkers for early disease diagnosis and prognosis. The significant challenges facing the available methods include improving the detection specificity and sensitivity in complex biological samples. Herein, a fluorescence assay was established based on a combination of immunomagnetic separation and a two-step signal amplification strategy for direct isolation and subsequent detection of exosomes. First, immunomagnetic beads capture and enrich the exosomes via antibody-antigen reactions. Second, bivalent cholesterol (BC) anchors spontaneously insert into the lipid bilayer of bead-captured exosomes, forming a "one to many" amplification effect. The simultaneous recognition of the surface protein and the lipid bilayer structure of the exosome significantly eliminates the interference risk from free proteins. The detection of exosomes converts to the detection of BC-anchors. Finally, the sticky end of the BC-anchor acts as the initiator to trigger the enzyme-free DNA circuits for secondary signal amplification. Under the optimal conditions, highly sensitive and selective detection of exosomes was achieved ranging from 5.5 x 10(3) to 1.1 x 10(7) particles/mu L with a limit of detection of 1.29 x 10(3) particles/mu L. Moreover, this method allows the isolation and quantitative analysis of exosomes in several biological fluids with satisfactory recovery rates (92.25-106.8%). Thus, this approach provides a sensitive, anti-interference platform for isolating and detecting exosomes.
资助项目	National Nature Science Foundation of China[21904078] ; National Nature Science Foundation of China[21976209] ; Taishan Scholar Project Special Funding[ts20190962] ; Key Project of Natural Science Foundation of Shandong Province[ZR2020KH030]
WOS关键词	EXTRACELLULAR VESICLES ; CANCER EXOSOMES ; CELL ; SECRETION ; PROTEINS ; BIOGENESIS ; PROGRESS
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000665642500016
资助机构	National Nature Science Foundation of China ; Taishan Scholar Project Special Funding ; Key Project of Natural Science Foundation of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29464]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Ma, Cuiping; Chen, Lingxin
作者单位	1.Qingdao Univ Sci & Technol, Shandong Prov Key Lab Biochem Engn, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China 2.Qingdao Chengyang Dist Peoples Hosp, Dept Hepatobiliary Surg, Qingdao 266109, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 4.Qufu Normal Univ, Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaokun,Shang, Hezhen,Ma, Cuiping,et al. A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification[J]. ANALYTICAL CHEMISTRY,2021,93(24):8493-8500.
APA	Wang, Xiaokun,Shang, Hezhen,Ma, Cuiping,&Chen, Lingxin.(2021).A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification.ANALYTICAL CHEMISTRY,93(24),8493-8500.
MLA	Wang, Xiaokun,et al."A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification".ANALYTICAL CHEMISTRY 93.24(2021):8493-8500.