Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians

doi:10.1016/j.cbpb.2021.110638

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物资源高效利用研究与发展中心

	Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians
	Ning, Junhao 1; Cao, Weian 2; Lu, Xia 1; Chen, Min 1; Liu, Bo 2; Wang, Chunde 1,2
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2021-10-01
卷号	256 页码:8
关键词	Argopecten irradians irradians Foxl2 Expression analysis RNA interference
ISSN号	1096-4959
DOI	10.1016/j.cbpb.2021.110638
通讯作者	Wang, Chunde(chundewang2007@163.com)
英文摘要	Transcription factor Foxl2 is an evolutionarily conserved gene playing pivotal roles in regulation of early ovarian differentiation and maintenance in animals. However, the Foxl2 gene has not been thoroughly studied in hermaphroditic scallops. In this study, we cloned and characterized a Foxl2 (designated as AiFoxl2) from the bay scallop Argopecten irradians irradians. The open reading frame of AiFoxl2 was 1122 bp encoding 373 amino acids residues and contained a conserved forkhead box domain. Quantitative real-time PCR showed that AiFoxl2 was mainly expressed in the ovary. Moreover, the highest expression of AiFoxl2 in the ovary was detected at proliferative stage and growing stage, while the lowest level was found at resting stage. During the embryonic and larval development, expression of AiFoxl2 was found first in fertilized eggs, increased significantly at the blastula stage, and reached peak value at the D-larvae stage. When AiFoxl2 was knocked down, testis development-related genes (Dmrt1, Sox7 and Sox9) were up-regulated significantly while the ovary development-related genes (Vg, HSD14, and GATA-1) were down-regulated manifestly. These findings suggested that AiFoxl2 was a femalerelated gene in A. i. irradians and may be involved in regulation of ovarian development and differentiation.
资助项目	National Natural Science Foundation of China[3197210450] ; National Natural Science Foundation of China[31972791] ; Scientific and Technological Project of Yantai, Shandong Province[2019LJRC141] ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences[ZR2017QD001]
WOS关键词	MOLECULAR-CLONING ; GENE-EXPRESSION ; DMRT1 ; DIFFERENTIATION ; MAINTENANCE ; TILAPIA ; TRAITS ; CELLS
WOS研究方向	Biochemistry & Molecular Biology ; Zoology
语种	英语
WOS记录号	WOS:000684852500021
资助机构	National Natural Science Foundation of China ; Scientific and Technological Project of Yantai, Shandong Province ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29655]
专题	海岸带生物资源高效利用研究与发展中心中国科学院烟台海岸带研究所
通讯作者	Wang, Chunde
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Ning, Junhao,Cao, Weian,Lu, Xia,et al. Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2021,256:8.
APA	Ning, Junhao,Cao, Weian,Lu, Xia,Chen, Min,Liu, Bo,&Wang, Chunde.(2021).Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,256,8.
MLA	Ning, Junhao,et al."Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 256(2021):8.