Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237

doi:10.15376/biores.15.4.8355-8368

	Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237
	Liu, Yunyun 1,2; Xie, Xianli 2; Liu, Wenhuan 2; Xu, Huijuan 1; Cao, Yunqi 2
刊名	BIORESOURCES
	2020-11-01
卷号	15 期号:4 页码:8355-8368
关键词	Clostridium thermocellum Consolidate bioprocess Bioethanol Sugarcane bagasse Fementer
ISSN号	1930-2126
DOI	10.15376/biores.15.4.8355-8368
通讯作者	Xu, Huijuan(xuhj@ms.giec.ac.cn)
英文摘要	A consolidated bioprocessing (CBP) using Clostridium thermocellum DSM 1237 for bioethanol production in anaerobic bottles and a 3-L fermenter from biomass was investigated. The effects of key operational parameters including different carbon sources, temperature, and substrates on the metabolic performance of the strain were firstly evaluated. It was found that ethanol yield reached 0.60 g/L with a cell biomass of 0.80 g/g at the optimal temperature of 60 degrees C with 0.5% (w/v) cellobiose. Further experiments indicated that sugarcane bagasse (SCB) could be utilized to efficiently culture this strain. Ethanol yield reached 0.68 g/L (65.8% of theoretical yield) from alkali-pretreated SCB. In the subsequent 3-L fermenter trial, the maximum ethanol 0.86 g/L (83.3% of theoretical yield) was achieved, with enzymes enriched in both cellulase and xylanase. The CBP provided enzymes on-site and integrated hydrolysis and fermentation in one-step, which might be an effective approach for economic bioethanol production.
资助项目	Program of Science & Technology Planning Project of Xi' an city[20193039YF027NS027] ; Natural Science Foundation of Shaanxi Province[2018JQ5134] ; National Natural Science Foundation of China[51876206] ; National Key Research and Development Program[2019YFB1503904] ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources[SKLCUSA-b201802] ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion[Y907k51001]
WOS关键词	CELLULOSIC ETHANOL
WOS研究方向	Materials Science
语种	英语
出版者	NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI
WOS记录号	WOS:000583802900074
资助机构	Program of Science & Technology Planning Project of Xi' an city ; Natural Science Foundation of Shaanxi Province ; National Natural Science Foundation of China ; National Key Research and Development Program ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources ; CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/34846]
专题	中国科学院广州能源研究所
通讯作者	Xu, Huijuan
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 2.Shaanxi Univ Sci & Technol, Coll Mech & Elect Engn, Xian 710021, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yunyun,Xie, Xianli,Liu, Wenhuan,et al. Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237[J]. BIORESOURCES,2020,15(4):8355-8368.
APA	Liu, Yunyun,Xie, Xianli,Liu, Wenhuan,Xu, Huijuan,&Cao, Yunqi.(2020).Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237.BIORESOURCES,15(4),8355-8368.
MLA	Liu, Yunyun,et al."Consolidated Bioprocess for Bioethanol Production from Lignocellulosic Biomass Using Clostridium thermocellum DSM 1237".BIORESOURCES 15.4(2020):8355-8368.