Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation | |
Yunhong Tan![]() | |
刊名 | In Vitro Cellular & Developmental Biology - Plant
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2013 | |
卷号 | x期号:x页码:x |
关键词 | Intermediate seeds Interspecific variation Intraspecies variation Cryotolerance Tropical seeds Germplasm conservation |
中文摘要 |
Several dehydration protocols were evaluated for their ability to cryopreserve intact seeds and excised embryonic axes of Mimusops elengi and Manilkara zapota (Sapotaceae). Both interspecific and intraspecific variations in cryotolerance were found. M. zapota embryonic axes were more tolerant of cryopreservation than those of M. elengi, and showed higher desiccation tolerance, higher post-thawing survival and development, and a much wider range of moisture contents for cryopreservation. Maximum development rates were 94% and 27% for M. zapota and M. elengi, respectively. Intact seeds of both species tolerated desiccation to low moisture levels, but were sensitive to liquid nitrogen exposure, and cryopreserved seeds failed to germinate. Assessment of developing embryos excised from cryopreserved seeds associated nonviability of cotyledons and plumules with germination failure. Other structures survived at variable rates; most hypocotyls and radicles (up to 76% and 98% for M. elengi and M. zapota, respectively) were viable. The different cryotolerance between hypocotyls and cotyledons is a critical cause for failure in cryopreservation, contributing to the difficulty in developing protocols for such intermediate oily seeds. |
公开日期 | 2013-10-08 |
内容类型 | 期刊论文 |
源URL | [http://ir.xtbg.org.cn/handle/353005/4687] ![]() |
专题 | 西双版纳热带植物园_支撑系统 |
推荐引用方式 GB/T 7714 | Yunhong Tan. Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation[J]. In Vitro Cellular & Developmental Biology - Plant,2013,x(x):x. |
APA | Yunhong Tan.(2013).Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation.In Vitro Cellular & Developmental Biology - Plant,x(x),x. |
MLA | Yunhong Tan."Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation".In Vitro Cellular & Developmental Biology - Plant x.x(2013):x. |
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