Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors

doi:10.1007/s11433-021-1752-0

CORC > 力学研究所 > 中国科学院力学研究所 > 非线性力学国家重点实验室

	Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors
	Li L(李龙)6,7; Hu, Jinglei 3,4,5; Wu, Huaping 2; Song F(宋凡)1,6,7
刊名	SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
	2021-10-01
卷号	64 期号:10 页码:10
关键词	cell adhesion ligand-ligand cis-interaction receptor-ligand interaction phase behavior
ISSN号	1674-7348
DOI	10.1007/s11433-021-1752-0
通讯作者	Hu, Jinglei(hujinglei@nju.edu.cn) ; Wu, Huaping(wuhuaping@gmail.com) ; Song, Fan(songf@lnm.imech.ac.cn)
英文摘要	Cell adhesion on a supported lipid bilayer (SLB) functionalized with ligand proteins is a widely-used cell-mimetic model for the study of the trans receptor-ligand interaction that mediates the adhesion, and has been shown to play an important role in unravelling the molecular players in cell adhesion. Experimental studies of such cell-SLB adhesion systems often assume that there is no cis interactions between the ligands. An important question remains whether the cis-interaction affects the trans receptor-ligand interaction. Using a statistical-mechanical model and Monte Carlo simulations with biologically relevant parameters, we find that the attractive cis-interaction of strength 1 k(B)T between adjacent ligands on the SLB can lead to an amplification of both the affinity and cooperativity of the receptor-ligand binding, thereby facilitating the phase separation within the adhering cell membrane. In contrast, the adhesion system is less sensitive to the repulsive cis-interaction between adjacent ligands on the SLB. Our results suggest that the ligand-ligand cis-interaction should be carefully considered in the cell-SLB adhesion experiments.
分类号	二类/Q1
资助项目	National Natural Science Foundation of China[11902327] ; National Natural Science Foundation of China[21973040] ; National Natural Science Foundation of China[11972041] ; Youth Innovation Promotion Association of Chinese Academy of Sciences, Strategic Priority Research Program of the Chinese Academy of Sciences[XDB22040102] ; Fundamental Research Funds for the Central Universities[14380228] ; Shenzhen Science and Technology Innovation Committee[JCYJ20200109150656717] ; Shenzhen Science and Technology Innovation Committee[JCYJ20170818110613113]
WOS关键词	MEMBRANE-ANCHORED RECEPTORS ; RAFTS ; DIFFUSION ; CONSTANTS ; CLUSTERS ; KINETICS ; MODELS
WOS研究方向	Physics
语种	英语
WOS记录号	WOS:000693207100001
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, Strategic Priority Research Program of the Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Shenzhen Science and Technology Innovation Committee
其他责任者	Hu, Jinglei ; Wu, Huaping ; Song, Fan
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/87408]
专题	力学研究所_非线性力学国家重点实验室
作者单位	1.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 2.Zhejiang Univ Technol, Coll Mech Engn, Hangzhou 310023, Peoples R China; 3.Nanjing Univ, Shenzhen Res Inst, Shenzhen 518057, Peoples R China; 4.Nanjing Univ, Inst Brain Sci, Nanjing 210023, Peoples R China; 5.Nanjing Univ, Kuang Yaming Honors Sch, Nanjing 210023, Peoples R China; 6.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; 7.Chinese Acad Sci, State Key Lab Nonlinear Mech LNM, Beijing 100190, Peoples R China;
推荐引用方式 GB/T 7714	Li L,Hu, Jinglei,Wu, Huaping,et al. Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2021,64(10):10.
APA	李龙,Hu, Jinglei,Wu, Huaping,&宋凡.(2021).Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,64(10),10.
MLA	李龙,et al."Cis-interaction of ligands on a supported lipid bilayer affects their binding to cell adhesion receptors".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 64.10(2021):10.