Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy

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	Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy
	Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Zhang CL(张常麟); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
刊名	CHINESE SCIENCE BULLETIN
	2013
卷号	58 期号:13 页码:1516-1519
关键词	atomic force microscopy CD20 rituximab lift scan force curve
ISSN号	1001-6538
通讯作者	刘连庆 ; 席宁 ; 张伟京
产权排序	1
中文摘要	Atomic force microscopy (AFM) was used to locate CD20 molecules on the surface of lymphoma Raji cells. Rituximab (a monoclonal antibody against CD20) molecules were linked onto the AFM tip via a polyethylene glycol (PEG) linker. Raji cells were adsorbed onto glass slides coated with poly-L-lysine. First, the CD20 distribution in a local area of the cell surface was visualized using the AFM lift scan mode. Second, 16 x 16 force curves were obtained from the same cell area to construct the CD20-rituximab binding force map. Finally, free rituximab was added to block the CD20 molecules on the cell surface and the lift phase image and CD20-rituximab force map were obtained again. The experimental results indicated that when the lift height was greater than the length of the PEG linker, no recognition sites were observed in the lift phase image. However, as the lift height decreased to the length of the PEG linker, some recognition sites were observed in the lift phase image and these sites were generally consistent with the pixels in the force map. After blocking, both the recognition sites in the lift phase image and the gray pixels in the binding force map decreased markedly. These results can improve our understanding of the distribution of protein molecules on the cell surface and facilitate further investigations into cellular functions.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	RECOGNITION EVENTS ; DNA-MOLECULES ; LIVING CELLS ; AFM ; SPECTROSCOPY ; LOCALIZATION ; NANOSCOPY ; REVEALS
收录类别	SCI
资助信息	National Natural Science Foundation of China [60904095, 61175103]; CAS
语种	英语
WOS记录号	WOS:000318527200005
公开日期	2013-10-05
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/12554]
专题	沈阳自动化研究所_机器人学研究室
推荐引用方式 GB/T 7714	Li M,Liu LQ,Xi N,et al. Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy[J]. CHINESE SCIENCE BULLETIN,2013,58(13):1516-1519.
APA	Li M.,Liu LQ.,Xi N.,Wang YC.,Zhang CL.,...&Zhang WJ.(2013).Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy.CHINESE SCIENCE BULLETIN,58(13),1516-1519.
MLA	Li M,et al."Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy".CHINESE SCIENCE BULLETIN 58.13(2013):1516-1519.