Ameliorating Ribosylation-Induced Amyloid-beta Pathology by Berberine via Inhibiting mTOR/p70S6K Signaling

doi:10.3233/JAD-200995

	Ameliorating Ribosylation-Induced Amyloid-beta Pathology by Berberine via Inhibiting mTOR/p70S6K Signaling
	Wang, Yang-Yang 1,4; Yan, Qian 3; Huang, Zhen-Ting 1,4; Zou, Qian 1,4; Li, Jing 1,4; Yuan, Ming-Hao 1,4; Wu, Liang-Qi 2,3; Cai, Zhi-You 1,4
刊名	JOURNAL OF ALZHEIMERS DISEASE
	2021
卷号	79 期号:2 页码:833-844
关键词	AGEs Alzheimer's disease amyloid-beta autophagy berberine mTOR
ISSN号	1387-2877
DOI	10.3233/JAD-200995
通讯作者	Wu, Liang-Qi(wulqi@ucas.ac.cn) ; Cai, Zhi-You(caizhiyou@ucas.ac.cn)
英文摘要	Background: Berberine (BBR) plays a neuroprotective role in the pathogenesis of Alzheimer's disease (AD), inhibiting amyloid-beta (A beta) production and promoting A beta clearance. Advanced glycation end products (AGEs) promote A beta aggregation and tau hyperphosphorylation. The activation of mTOR signaling occurring at the early stage of AD has a prominent impact on the A beta production. This work focused on whether BBR regulates the production and clearance of ribosylation-induced A beta pathology via inhibiting mTOR signaling. Objective: To explore whether BBR ameliorates ribosylation-induced A beta pathology in APP/PS1 mice. Methods: Western blot and immunofluorescence staining were used to detect the related proteins of the mammalian target of Rapamycin (mTOR) signaling pathway and autophagy, as well as the related kinases of A beta generation and clearance. Tissue sections and Immunofluorescence staining were used to observe A beta(42) in APP/PS1 mice hippocampal. Morris water maze test was used to measure the spatial learning and memory of APP/PS1 mice. Results: BBR improves spatial learning and memory of APP/PS1 mice. BBR limits the activation of mTOR/p70S6K signaling pathway and enhances autophagy process. BBR reduces the activity of BACE1 and y-secretase induced by D-ribose, and enhances A beta-degrading enzymes and Neprilysin, and inhibits the expression of A beta in APP/PS1 mice. Conclusion: BBR ameliorates ribosylation-induced A beta pathology via inhibiting mTOR/p70S6K signaling and improves spatial learning and memory of the APP/PS1 mice.
资助项目	Chongqing Natural Science Foundation[cstc2018jcyjAX0602] ; Science andTechnology Planning Project ofYuzhong District of Chongqing[20180104] ; Medical Science and Technology Innovation Fund project of Chongqing General Hospital[Y2018MSXM03] ; Chongqing Health and Family Planning Scientific Research Project[ZY201702042]
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	IOS PRESS
WOS记录号	WOS:000611560100029
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/12928]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Wu, Liang-Qi; Cai, Zhi-You
作者单位	1.Univ Chinese Acad Sci, Chongqing Gen Hosp, Dept Neurol, 312 Zhongshan First Rd, Chongqing 400013, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, 266 Fangzheng Ave, Chongqing 400714, Peoples R China 4.Chongqing Key Lab Neurodegenerat Dis, Chongqing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yang-Yang,Yan, Qian,Huang, Zhen-Ting,et al. Ameliorating Ribosylation-Induced Amyloid-beta Pathology by Berberine via Inhibiting mTOR/p70S6K Signaling[J]. JOURNAL OF ALZHEIMERS DISEASE,2021,79(2):833-844.
APA	Wang, Yang-Yang.,Yan, Qian.,Huang, Zhen-Ting.,Zou, Qian.,Li, Jing.,...&Cai, Zhi-You.(2021).Ameliorating Ribosylation-Induced Amyloid-beta Pathology by Berberine via Inhibiting mTOR/p70S6K Signaling.JOURNAL OF ALZHEIMERS DISEASE,79(2),833-844.
MLA	Wang, Yang-Yang,et al."Ameliorating Ribosylation-Induced Amyloid-beta Pathology by Berberine via Inhibiting mTOR/p70S6K Signaling".JOURNAL OF ALZHEIMERS DISEASE 79.2(2021):833-844.