Gut bacteria Vibrio sp. and Aeromonas sp. trigger the expression levels of proinflammatory cytokine: First evidence from the germ-free zebrafish

doi:10.1016/j.fsi.2020.08.018

	Gut bacteria Vibrio sp. and Aeromonas sp. trigger the expression levels of proinflammatory cytokine: First evidence from the germ-free zebrafish
	Xin, Guang-Yuan 1,2; Li, Wei-Guo 2; Suman, Thodhal Yoganandham 1,2; Jia, Pan-Pan 1; Ma, Yan-Bo 1; Pei, De-Sheng 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-11-01
卷号	106 页码:518-525
关键词	Zebrafish Nod-like receptors Toll-like receptors Aeromonas sp. Vibrio sp
ISSN号	1050-4648
DOI	10.1016/j.fsi.2020.08.018
通讯作者	Pei, De-Sheng(peids@cigit.ac.cn)
英文摘要	Gut microbiota plays a central part in the regulation of multiple host metabolic pathways, such as homeostasis, immunostasis, mucosa permeability, and even brain development. Though, slight known about the function of an individual gut bacterium in zebrafish. In this study, germ-free (GF) and conventionally reared (CV) zebrafish models utilized for studying the role of gut bacteria Vibrio sp. and Aeromonas sp. After the analysis of gut microbial profile in zebrafish male and female at three-month age, Proteobacteria and Fusobacteria dominated the main composition of zebrafish intestinal microflora. However, the relative richness of them was different base on gender variance. Aeromonas sp. and Vibrio sp. belonging to Proteobacteria phylum of bacteria were isolated from zebrafish gut, and their potential capacities to trigger innate immunity were investigated. In gut microbiota absence, the expression levels of the innate immunity genes in the GF group were not significantly changed compared to the CV group. After exposure to Aeromonas sp. and Vibrio sp., the expression levels of myd88, TLRs-, and inflammation-related genes were increased in both GF and CV groups, except tlr2 and NLRs-related genes. However, the expression level of NF-kappa B and JNK/AP-1 pathway genes were all decreased after exposure to Aeromonas sp. and Vibrio sp. in both GF and CV groups. Interestingly, inflammation-related genes (tnfa, tnfb, and il1 beta) were activated in the CV group, and there were not significantly changed in the GF group, indicating that other bacteria were indispensable for Aeromonas sp. or Vibrio sp. to activate the inflammation response. Taken together, this is the first study of gut bacteria Vibrio sp. and Aeromonas sp. prompting the innate immune response using the GF and CV zebrafish model.
资助项目	Chongqing Key Program of Basic Research and Advanced Exploration Project[cstc2019jcyj-zdxmX0035] ; CAS Team Project of the Belt and Road ; Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing[CSTCCXLJRC201714] ; Program of China-Sri Lanka Joint Research and Demonstration Center for Water Technology by Chinese Academy of Sciences ; China-Sri Lanka Joint Center for Education and Research by Chinese Academy of Sciences ; China Youth Innovation Program of Chongqing Institute of CAS[Y83A160S10]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000579494400052
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/11916]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Pei, De-Sheng
作者单位	1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China
推荐引用方式 GB/T 7714	Xin, Guang-Yuan,Li, Wei-Guo,Suman, Thodhal Yoganandham,et al. Gut bacteria Vibrio sp. and Aeromonas sp. trigger the expression levels of proinflammatory cytokine: First evidence from the germ-free zebrafish[J]. FISH & SHELLFISH IMMUNOLOGY,2020,106:518-525.
APA	Xin, Guang-Yuan,Li, Wei-Guo,Suman, Thodhal Yoganandham,Jia, Pan-Pan,Ma, Yan-Bo,&Pei, De-Sheng.(2020).Gut bacteria Vibrio sp. and Aeromonas sp. trigger the expression levels of proinflammatory cytokine: First evidence from the germ-free zebrafish.FISH & SHELLFISH IMMUNOLOGY,106,518-525.
MLA	Xin, Guang-Yuan,et al."Gut bacteria Vibrio sp. and Aeromonas sp. trigger the expression levels of proinflammatory cytokine: First evidence from the germ-free zebrafish".FISH & SHELLFISH IMMUNOLOGY 106(2020):518-525.