Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor

doi:10.1039/d0ra05083k

	Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor
	Wang, Sen 2,3; Liang, Liyuan 2,3; Tang, Jing 2,3; Cai, Yao 1,2; Zhao, Chuanqi 3,4; Fang, Shaoxi 2,3; Wang, Huabin 2,3; Weng, Ting 2,3; Wang, Liang 2,3; Wang, Deqiang 2,3
刊名	RSC ADVANCES
	2020-07-20
卷号	10 期号:45 页码:27215-27224
DOI	10.1039/d0ra05083k
通讯作者	Liang, Liyuan(liangliyuan@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn)
英文摘要	Telomere sequences can spontaneously form G-quadruplexes (G4) in the presence of some cations. In view of their relevance to genetic processes and potential as therapeutic-targets, hitherto, a wealth of conventional techniques have been reported for interrogation of G4 conformation diversity and corresponding folding kinetics, most of which are limited in precision and sensitivity. This work introduces a label-free solid-state nanopore (SSN) approach for the determination of inter-, intra- and tandem molecular G4 with distinct base permutation in various cation buffers with a tailored aperture and meanwhile captures the single-molecule G4 folding process. SSN translocation properties elucidated that both inter- and intramolecular G4 generated higher current blockage with longer duration than flexible homopolymer nucleotide, and intramolecular G4 are structurally more stable with higher event frequency and longer blockage time than intermolecular ones; base arrangement played weak role in translocation behaviors; the same sequences with one, two and three G4 skeletons displayed an increase in current blockage and a gradual extension in dwell time with the increase of molecule size recorded in the same nanopore. We observed the conformation change of single-molecule G4 which indicated the existence of folding/unfolding equilibration in nanopore, and real-time test suggested a gradual formation of G4 with time. Our results could provide a continued and improved understanding of the underlying relevance of structural stability and G4 folding process by utilizing SSN platform which exhibits strategic potential advances over the other methods with high spatial and temporal resolution.
资助项目	Natural Science Foundation of Chongqing, China[cstc2017jcyjB0105] ; Youth Innovation Promotion Association[2017392] ; Pioneer Hundred Talents Program of the Chinese Academy of Sciences ; Instrumentation Development Program of the Chinese Academy of Sciences[YZ201568] ; University of Chinese Academy of Sciences
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000555612200062
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/11654]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Liang, Liyuan; Wang, Deqiang
作者单位	1.Jilin Univ, Coll Instrument Sci & Elect Engn, Changchun 130016, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China 3.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 4.Chinese Acad Sci, Changchun Inst Appl Chem, Lab Chem Biol, State Key Lab Rare Earth Resource Utilizat, Changchun 130022, Jilin, Peoples R China
推荐引用方式 GB/T 7714	Wang, Sen,Liang, Liyuan,Tang, Jing,et al. Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor[J]. RSC ADVANCES,2020,10(45):27215-27224.
APA	Wang, Sen.,Liang, Liyuan.,Tang, Jing.,Cai, Yao.,Zhao, Chuanqi.,...&Wang, Deqiang.(2020).Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor.RSC ADVANCES,10(45),27215-27224.
MLA	Wang, Sen,et al."Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor".RSC ADVANCES 10.45(2020):27215-27224.