Functionalized magnetic mesoporous silica nanoparticles: Fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth

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	Functionalized magnetic mesoporous silica nanoparticles: Fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth
	Wang, Feng 1; Huang, Wei 2; Guo, Chen 1; Liu, Chun-Zhao 1
刊名	BIORESOURCE TECHNOLOGY
	2012-12-01
卷号	126 期号:0 页码:117-122
关键词	Mesoporous Size-selective Purification Laccase Magnetic separation
ISSN号	0960-8524
通讯作者	Liu, CZ
英文摘要	A simple and highly efficient protocol using magnetic mesoporous silica nanoparticles (MMSNPs) with metal affinity ligands was developed to directly capture laccase from Trametes versicolor fermentation broth. The Cu2+-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu2+) with pore sizes ranging from 3.6 to 27.1 nm exhibited size selectivity on laccase capture from the fermentation broth, and the MMSNPs-Cu2+ with an average pore size of 14.5 nm provided 60.6-fold purification of laccase and 114.6% recovery yield of enzyme activity. Both size selectivity of the MMSNPs and affinity of the chelated metal ion resulted in high laccase capture efficiency from the fermentation broth. The most efficient MMSNPs-Cu2+ demonstrated no significant loss in laccase capture effectiveness following 10 reuse cycles. This simple and efficient strategy has the potential to be used for the robust and inexpensive preparation of purified laccase at the industrial scale. (C) 2012 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	WORMHOLE FRAMEWORK STRUCTURES ; PROTEIN SEPARATION ; PURIFICATION ; IMMOBILIZATION ; MESOSTRUCTURE ; ENRICHMENT ; SPHERES ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000313997500017
公开日期	2013-09-30
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/2687]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Res Inst Nutr Resources, Beijing 100069, Peoples R China
推荐引用方式 GB/T 7714	Wang, Feng,Huang, Wei,Guo, Chen,et al. Functionalized magnetic mesoporous silica nanoparticles: Fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth[J]. BIORESOURCE TECHNOLOGY,2012,126(0):117-122.
APA	Wang, Feng,Huang, Wei,Guo, Chen,&Liu, Chun-Zhao.(2012).Functionalized magnetic mesoporous silica nanoparticles: Fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth.BIORESOURCE TECHNOLOGY,126(0),117-122.
MLA	Wang, Feng,et al."Functionalized magnetic mesoporous silica nanoparticles: Fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth".BIORESOURCE TECHNOLOGY 126.0(2012):117-122.