Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay

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	Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay
	Wan, Yi 1,2; Qi, Peng1,2 ; Zhang, Dun 1; Wu, Jiajia1,2 ; Wang, Yi1
刊名	BIOSENSORS & BIOELECTRONICS
	2012-03-15
卷号	33 期号:1 页码:69-74
关键词	Enzyme-linked immunosorbent assay Manganese Oxide Bacteria detection Nanomaterials Enzyme mimetics
ISSN号	0956-5663
通讯作者	Zhang, D (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Nanomaterial-based enzyme-linked immunosorbent assay (ELISA) with sufficient sensing specificity is a useful analytical tool for the detection of toxicologically important substances in complicated biological systems. Increasing worldwide demand for nanomaterials and increasing concern on their safe development and use, require a simple, stable, and sensitive detection assay for pathogen evaluation and environmental monitoring. However, this goal is not yet achieved. A design for a hybrid MnO2 nanowire-ELISA using the sandwich assay format, which provides quantitative binding information for both a specific antibody and the pathogen, sulfate-reducing bacteria, and detects pathogen concentration, is presented. 3,3',5,5'-Tetramethylbenzidine was used as the substrate and was allowed to react with the MnO2 nanowires without H2O2 in the reaction system. The kinetic parameters were measured with the system acting as a catalytic biosensor. The effectiveness of the MnO2 nanowire-based biosensor was demonstrated by its sensitive detection of the pathogen. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
收录类别	SCI
原文出处	10.1016/j.bios.2011.12.033
语种	英语
WOS记录号	WOS:000301699800011
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12474]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wan, Yi,Qi, Peng,Zhang, Dun,et al. Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay[J]. BIOSENSORS & BIOELECTRONICS,2012,33(1):69-74.
APA	Wan, Yi,Qi, Peng,Zhang, Dun,Wu, Jiajia,&Wang, Yi.(2012).Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay.BIOSENSORS & BIOELECTRONICS,33(1),69-74.
MLA	Wan, Yi,et al."Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay".BIOSENSORS & BIOELECTRONICS 33.1(2012):69-74.