QQ客服 官方微博 反馈留言

题名	发酵法生产1，3-丙二醇工艺的研究
作者	朱丙田
学位类别	硕士
答辩日期	2000-06
授予单位	中国科学院研究生院
导师	刘德华
关键词	1，3-丙二醇 甘油发酵 克伯雷氏肺炎杆菌 辅助发酵
其他题名	Study on the Technology of 1,3-Propanediol Production by Fermentation
学位专业	生物化工
中文摘要	本论文主要研究了克伯雷氏肺炎杆菌PK2发酵生产1，3-丙二醇的工艺。从已有的三株菌中筛选了一株能生产1，3-丙二醇且性能良好的菌株——克伯雷氏肺炎杆菌PK2，确定了有氧培养种子、厌氧发酵生产1，3-丙二醇的工艺。较优的种子培养时间为18小时左右，接种量为10%。研究了温度和pH对发酵的影响，结果表明在30℃左右有利于细胞的生长，虽然37℃时细胞生长较慢，但有利于提高甘油的转化率；弱酸性环境对细胞生长和甘油消耗有利，但甘油的转化率不高，pH为7时甘油转化率最高。酵母粉是1，3-丙二醇发酵所必需的营养成份，能显著地影响细胞的生长代谢。Co~(2+)是构成甘油脱水酶辅酶的必需因子，影响着甘油的代谢速率，研究表明0.58mg/l Co~(2+)对发酵最为有利。在研究培养基中碳氮磷单因子对发酵影响的基础上，进一步利用正交实验确定了碳氮磷的最佳配方为：甘油5%，NH_4Cl 5.35g/l, NaH_2PO_4·2H_2O 3.2g/l。另外，实验还发现氮磷存在着交互作用。在用葡萄糖和甘油同时作为克伯雷氏肺炎杆菌生长的混合底物时，细胞优先利用葡萄糖。葡萄糖作为辅助底物虽然有利于细胞生长，但影响着甘油的消耗及其转化率，从发酵结果来看，1，3-丙二醇氧化还原充分利用细胞代谢葡萄糖产生的NADH，使甘油转化率达不到理论值（100%）。较低的葡萄糖浓度（0.5-1%）可以提高甘油的转化率，使甘油转化率最高可达58.9%；较高的葡萄糖浓度对甘油的转化率不再具有明显的促进作用。
英文摘要	Bioconversion from glycerol to 1,3-propanediol by fermentation with Klebsiella pneumoniae PK2 was studied in this paper. A high-1,3-propanediol-productivity stain-Klebsiella pneumoniae PK2 was selected from three stains of bacteria gained in our lab. An optimal technological process was determined as follows: 1) Inoculum aerobic condition, cultural time 18 hours, inoculum ratio 10% (v/v) .2) Bioconversion at anaerobic fermentation. With the studies on temperature and pH. 30 ℃ was favorable to cell growth and 37 ℃ was optimal bioconversion temperature for anaerobic fermentation; neuter or subacidic conditions were favorable to cell growth and glycerol consumption, but not best to 1,3-propanediol production, while pH 7 was best to convert glycerol to 1,3-propanediol. Yeast extract was found to have significant importance to glycerol fermentation, which impacted on cell growth and glycerol metabolism. Co~(2+) is a necessary part of Coenzyme B_(12)-dependent glycerol dehydratases, which greatly affected glycerol metabolism, and 0.58mg/l was optimal concentration of Co~(2+) to 1,3-propanediol production. The effect of single factors-C, N, P on the fermentation was also studied. Furthermore, the orthogonal experiments were carried out based on the above single factor experimental results. The optimal concentrations of C、 N、 P in medium were as follows: 50g/l glycerol, NH_4Cl 5.35g/l, NaH_2PO_4·2H_2O 3.2g/l, under which the glycerol convers ion ratio was 46.7%(mol/mol). In addition, it is showed from the result of experiments that interaction of carbon and phosphor occurred in the fermentation. While Klebsiella pneumoniae PK2 grown on glycerol-glucose mixtures, cell first consumed glycerol. As additional substrate, glucose was advantageous to cell growth, but it affected glycerol consumption and glycerol convers ion ratio. From the experimental results, 1,3-propanediol oxidoreductase didn't adequately utilized NADH from glucose metabolism, which couldn't improve glycerol convers ion ratio to 100%. Lower glucose (0.5-1%) would enhance glycerol convers ion ratio, which best access to 58.9%, But higher glucose couldn't obviously accelerate gltcerol convers ion ratio.
语种	中文
公开日期	2013-09-26
页码	83
内容类型	学位论文
源URL	[http://ir.ipe.ac.cn/handle/122111/1946]
专题	过程工程研究所_研究所（批量导入）
推荐引用方式 GB/T 7714	朱丙田. 发酵法生产1，3-丙二醇工艺的研究[D]. 中国科学院研究生院. 2000.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。