The Impact of Exogenous Aerobic Bacteria on Sustainable Methane Production Associated with Municipal Solid Waste Biodegradation: Revealed by High-Throughput Sequencing

doi:10.3390/su12051815

	The Impact of Exogenous Aerobic Bacteria on Sustainable Methane Production Associated with Municipal Solid Waste Biodegradation: Revealed by High-Throughput Sequencing
	Ge, Sai 3,4,5; Ma, Jun 1,2,5; Liu, Lei 1,2,5; Yuan, Zhiming 3
刊名	SUSTAINABILITY
	2020-03-01
卷号	12 期号:5 页码:11
关键词	anaerobic digestion municipal solid waste microbial communities exogenous aerobic bacteria methane production methanogenic bacteria
DOI	10.3390/su12051815
英文摘要	In this work, the impact of exogenous aerobic bacteria mixture (EABM) on municipal solid waste (MSW) is well evaluated in the following aspects: biogas production, leachate analysis, organic waste degradation, EABM population, and the composition of microbial communities. The study was designed and performed as follows: the control bioreactor (R1) was filled up with MSW and the culture medium of EABM and the experimental bioreactor (R2) was filled up with MSW and EABM. The data suggests that the composition of microbial communities (bacterial and methanogenic) in R1 and R2 were similar at day 0, while the addition of EABM in R2 led to a differential abundance of Bacillus cereus, Bacillus subtilis, Staphylococcus saprophyticus, Staphlyoccus xylosus, and Pantoea agglomerans in two bioreactors. The population of exogenous aerobic bacteria in R2 greatly increased during hydrolysis and acidogenesis stages, and subsequently increased the degradation of volatile solid (VS), protein, lipid, and lignin by 59.25%, 25.68%, 60.47%, and 197.62%, respectively, compared to R1. The duration of hydrolysis and acidogenesis in R2 was 33.33% shorter than that in R1. At the end of the study, the accumulative methane yield in R2 (494.4 L) was almost three times more than that in R1 (187.4 L). In addition, the abundance of acetoclasic methanogens increased at acetogenesis and methanogenesis stages in both bioreactors, which indicates that acetoclasic methanogens (especially Methanoseata) could contribute to methane production. This study demonstrates that EABM can accelerate organic waste degradation to promote MSW biodegradation and methane production. Moreover, the operational parameters helped EABM to generate 20.85% more in accumulative methane yield. With a better understanding of how EABM affects MSW and the composition of bacterial community, this study offers a potential practical approach to MSW disposal and cleaner energy generation worldwide.
资助项目	National Natural Science Foundation of China[41977254] ; Youth Innovation Promotion Association CAS[2017376] ; Foundation for Innovative Research Groups of Hubei Province[2019CFA012] ; Wuhan Science and Technology Conversion Special Project[2018060403011348]
WOS研究方向	Science & Technology - Other Topics ; Environmental Sciences & Ecology
语种	英语
出版者	MDPI
WOS记录号	WOS:000522470900116
内容类型	期刊论文
源URL	[http://119.78.100.198/handle/2S6PX9GI/23911]
专题	中科院武汉岩土力学所
通讯作者	Liu, Lei; Yuan, Zhiming
作者单位	1.Hubei Prov Key Lab Contaminated Sludge & Soil Sci, Wuhan 430071, Peoples R China 2.IRSM CAS HK PolyU Joint Lab Solid Waste Sci, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens, Wuhan 430071, Peoples R China 4.Shanxi Datong Univ, Sch Chem & Environm Engn, Datong 037009, Peoples R China 5.Chinese Acad Sci, Inst Rock & Soil Mech, State Key Lab Geomech & Geotech Engn, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Ge, Sai,Ma, Jun,Liu, Lei,et al. The Impact of Exogenous Aerobic Bacteria on Sustainable Methane Production Associated with Municipal Solid Waste Biodegradation: Revealed by High-Throughput Sequencing[J]. SUSTAINABILITY,2020,12(5):11.
APA	Ge, Sai,Ma, Jun,Liu, Lei,&Yuan, Zhiming.(2020).The Impact of Exogenous Aerobic Bacteria on Sustainable Methane Production Associated with Municipal Solid Waste Biodegradation: Revealed by High-Throughput Sequencing.SUSTAINABILITY,12(5),11.
MLA	Ge, Sai,et al."The Impact of Exogenous Aerobic Bacteria on Sustainable Methane Production Associated with Municipal Solid Waste Biodegradation: Revealed by High-Throughput Sequencing".SUSTAINABILITY 12.5(2020):11.