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题名	压力脉动穿流强制通风固态发酵的研究及应用
作者	赵华
学位类别	博士
答辩日期	2002
授予单位	中国科学院过程工程研究所
授予地点	中国科学院过程工程研究所
导师	李佐虎
关键词	固态发酵 压力脉动 压力脉动穿流强制通风 纤维素酶 生物肥料
其他题名	Study on pressure oscillation coupled with forced aeration through the medium in solid state fermentation
学位专业	生物化工
中文摘要	固态发酵工业化的瓶颈是反应器,如何解决供氧和传热又是其技术的关键.围绕此问题,该文进行了以下几方面的研究:首先,采用改进Na<,2>SO<,3>法和对固体培养基内温度的测定,对已有的压力脉动固态发酵反应器中的供氧、传热问题进行了系统研究.为进一步强化压力脉动固态发酵反应器的传热效果,提出了对固态发酵中气相控制的新方法:压力脉动穿流强制通风,并设计出压力脉动穿流强制通风固态发酵反应器.然后,分别以草泥炭和秸杆为基质,对固氮菌的压力脉动固态发酵及压力脉动穿流强制通风固态发酵新工艺作了初步研究.通过对压力脉动的振幅、频率及强制通风量等工艺条件的探讨,使得A.vinelandii、Rhizobiumleguminosarum活菌数分别高达770亿/g和530亿/g.同液态发酵相比，经过压力脉动固态发酵的生物肥料具有起始活菌数多的优点，且保存90天,活菌数依然高达22、34亿/g.最后,主要从反应器内部结构上,对压力脉动穿流强制通风固态发酵反应器工程放大提出了初步结构设想.
英文摘要	It is well known that the oxygen and heat transfer are the key problems in large-scale solid-state fermentation systems. Our efforts are therefore focused on these problems and find feasible solution to solve it. Firstly, the transport phenomena in the periodic pressure oscillating, solid-state bioreactor (PPB) were investigated in detail by using the modified sulfite method and measuring the temperature in the fermenting solids. At 6 cm, 0.35 MPa, the maximal oxygen transfer rate reached 487 mmol kg'1 h"1 in the PPB, compared with 37 mmol kg"1 h"1 in conventional static tray systems. Also, the maximal temperature reached 31.4°C and 33.8°C, respectively, while 34.7°C and 36.8°C in conventional static tray fermentation of Azotobacter vinelandii and Penicillium decumbens. Clearly, periodic oscillation in air pressure significantly enhanced oxygen and heat transfer in the PPB. Then, to further improvement of heat transfer in the PPB, a novel control of air pressure, periodic pressure oscillation coupled with forced aeration through the medium, was proposed. As a result, the novel solid state bioreactor, periodic pressure oscillation coupled with forced aeration, solid state bioreactor—PPCFB, based on the novel idea mentioned above was designated. The technique was shown to be effective in the control of temperature in the fermenting solids. At 10 cm, 0.3 MPa, and 5 1 min"1, the temperature in the fermenting medium reached 34.2°C after 96 h, while 44.7°C in conventional static tray systems. Additionally, the results of its evaluation for enzyme formation by P. decumbens indicated that the maximal FPA and P -Gase, reached 9.08 IU g"1 and 41.64 IU g"1, respectively, which was about a 1.5-2 fold increased cellulase production compared with conventional tray bioreactors. Attempts were also made in using Venturi tube as a inoculum system. Effects of inoculum culture time and airflow rate as well as bed depth on the viable cells/spores distribution were investigated. At 6 cm, 15 1 min'1, and the inolocum culture time of 14 h, the viable cells/spores were easily distributed in the substrate. Later, attempts were made using charcoal and wheat straw as inert supports for biofertilizer production in the PPB as well as PPCFB. A. vinelandii and Rhizobium leguminosarum were fermented and subsequently reached 7.7X 1010 cfu g"1 and 5.3 X 1010 cfu g"1 substrate dry matter, respectively, in this system. Compared with submerged fermentation, the initial viable cells in the products via PPB were higher. Consequently, the viable cells reached 2.2 X109 cfu g"1 and 3.4 X109 cfu g"1 substrate dry matter, respectively, after 90 days stock. In the end, the scale-up strategies for periodic pressure oscillation coupled with forced aeration through the medium, solid-state fermentation systems were proposed.
语种	中文
公开日期	2013-09-16
页码	132
内容类型	学位论文
源URL	[http://ir.ipe.ac.cn/handle/122111/1352]
专题	过程工程研究所_研究所（批量导入）
推荐引用方式 GB/T 7714	赵华. 压力脉动穿流强制通风固态发酵的研究及应用[D]. 中国科学院过程工程研究所. 中国科学院过程工程研究所. 2002.

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