A facile two-column chromatographic process for efficient purification of paclitaxel from crude extract

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	A facile two-column chromatographic process for efficient purification of paclitaxel from crude extract
	Sun, HH ; Li, XN ; Ma, GH ; Su, ZG ; Li, XN
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2005
卷号	28 期号:4 页码:605-617
关键词	crude extract of Taxus spp. paclitaxel 10-deacetyl-7-epi-paclitaxel preparative low-pressure chromatography purification
ISSN号	1082-6076
英文摘要	A facile, preparative low-pressure chromatographic process was developed for purification of paclitaxel from crude extract of Taxus spp. The process consisted of normal-phase and a reversed-phase chromatography without utilization of any liquid-liquid partition step. The normal-phase chromatography was designed to separate paclitaxel from a majority of unwanted compounds to obtain an initial purification. Silica gel and Al2O3 were compared as packing materials for this step. The result demonstrated that basic Al2O3 was better than silica gel to give a higher throughput. A further advantage of the Al2O3 was its ability of removing 10-deacetyl-7-epipaclitaxel, a compound difficult to be separated from the paclitaxel in subsequent purification. The chromatographic fraction of Al2O3 was purified by a subsequent reversed-phase column chromatography, employing a novel uniform porous microsphere, named PST, as the packing material. Combination of the two steps of column chromatography and one step of crystallization was able to purify paclitaxel, from 0.46% to more than 98% with the total recovery of 71%. This integrated chromatographic procedure was reproducible, efficient, and simple to use.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	LIQUID-CHROMATOGRAPHY ; TAXOL
收录类别	SCI
语种	英语
WOS记录号	WOS:000227179600010
公开日期	2013-09-20
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/1579]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Beijing Univ Chem Technol, Sch Chem Engn, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Sun, HH,Li, XN,Ma, GH,et al. A facile two-column chromatographic process for efficient purification of paclitaxel from crude extract[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2005,28(4):605-617.
APA	Sun, HH,Li, XN,Ma, GH,Su, ZG,&Li, XN.(2005).A facile two-column chromatographic process for efficient purification of paclitaxel from crude extract.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,28(4),605-617.
MLA	Sun, HH,et al."A facile two-column chromatographic process for efficient purification of paclitaxel from crude extract".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 28.4(2005):605-617.