Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)

	Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)
	Lin, Fan 1,2; Liu, Qinghua 1; Li, Mingyou 3; Li, Zhendong 3; Hong, Ni 3; Li, Jun 1; Hong, Yunhan 3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
	2012
卷号	8 期号:6 页码:882-890
关键词	Pagrus major PGCs vasa transgene GFP
ISSN号	1449-2288
通讯作者	Li, J (reprint author), Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China.
英文摘要	Primordial germ cells (PGCs) are the precursors of gametes responsible for genetic transmission to the next generation. They provide an ideal system for cryopreservation and restoration of biodiversity. Recently, considerable attention has been raised to visualize, isolate and transplant PGCs within and between species. In fish, stable PGC visualization in live embryo and individual has been limited to laboratory fish models such as medaka and zebrafish. One exception is the rainbow trout, which represents the only species with aquaculture importance and has GFP-labeled germ cells throughout development. PGCs can be transiently labeled by embryonic injection of mRNA containing green fluorescence protein gene (GFP) and 3'-untranslated region (3'-UTR) of a maternal germ gene such as vasa, nos 1, etc. Stable PGC labeling can be achieved through production of transgenic animals by some transcriptional regulatory sequences from germ genes, such as the vasa promoter and 3'-UTR. In this study, we reported the functional analyses of the red seabream vasa (Pmvas) regulatory sequences, using medaka as a model system. It was showed that injection of GFP-Pmvas3'UTR mRNA was able to label medaka PGCs during embryogenesis. Besides, we have constructed pPmvasGFP transgenic vector, and established a stable transgenic medaka line exhibiting GFP expression in germ cells including PGCs, mitotic and meiotic germ cells of both sexes, under control of the Pmvas transcriptional regulatory sequences. It is concluded that the Pmvas regulatory sequences examined in this study are sufficient for germ cell expression and labeling.
学科主题	Biochemistry & Molecular Biology ; Biology
收录类别	SCI
原文出处	10.7150/ijbs.4421
语种	英语
WOS记录号	WOS:000306062300010
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12254]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
推荐引用方式 GB/T 7714	Lin, Fan,Liu, Qinghua,Li, Mingyou,et al. Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2012,8(6):882-890.
APA	Lin, Fan.,Liu, Qinghua.,Li, Mingyou.,Li, Zhendong.,Hong, Ni.,...&Hong, Yunhan.(2012).Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major).INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,8(6),882-890.
MLA	Lin, Fan,et al."Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 8.6(2012):882-890.