Three ferritin subunits involved in immune defense from bay scallop Argopecten irradians

	Three ferritin subunits involved in immune defense from bay scallop Argopecten irradians
	Li, Juan 1,2; Li, Li 1; Zhang, Shoudu 1,2; Li, Jiale 3,4; Zhang, Guofan 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-02-01
卷号	32 期号:2 页码:368-372
关键词	Argopecten irradians Ferritin Iron-withholding strategy Gene structure Immune response
ISSN号	1050-4648
通讯作者	Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Ferritin is a ubiquitous protein that plays an important role in iron storage and iron-withholding strategy of innate immunity. In this study, three genes encoding different ferritin subunits were cloned from bay scallop Argopecten irradians (AiFer1, AiFer2 and AiFer3) by rapid amplification of cDNA ends (RACE) approaches based on the known ESTs. The open reading frames of the three ferritins are of 516 bp, 522 bp and 519 bp, encoding 171,173 and 172 amino acids, respectively. All the AiFers contain a putative Iron Regulatory Element (IRE) in their 5'-untranslated regions. The deduced amino acid sequences of AiFers possess both the ferroxidase center of mammalian H ferritin and the iron nucleation site of mammalian L ferritin. Gene structure study revealed two distinct structured genes encoding a ferritin subunit (AiFer3). Quantitative real-time PCR analysis indicated the significant up-regulation of AiFers in hemocytes after challenged with Listonella anguillarum, though the magnitudes of AiFer1 and AiFer2 were much higher than that of AiFer3. Taken together, these results suggest that AiFers are likely to play roles in both iron storage and innate immune defense against microbial infections. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2011.10.030
语种	英语
WOS记录号	WOS:000300751800017
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12253]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China 4.Shanghai Univ, Inst E, Aquaculture Div, Shanghai 200041, Peoples R China
推荐引用方式 GB/T 7714	Li, Juan,Li, Li,Zhang, Shoudu,et al. Three ferritin subunits involved in immune defense from bay scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(2):368-372.
APA	Li, Juan,Li, Li,Zhang, Shoudu,Li, Jiale,&Zhang, Guofan.(2012).Three ferritin subunits involved in immune defense from bay scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,32(2),368-372.
MLA	Li, Juan,et al."Three ferritin subunits involved in immune defense from bay scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 32.2(2012):368-372.