Germ cells and fertilization differences among Japanese flounder Paralichthys olivaceus, summer flounder Paralichthys dentatus and their first and second generations

	Germ cells and fertilization differences among Japanese flounder Paralichthys olivaceus, summer flounder Paralichthys dentatus and their first and second generations
	Sui, J.1,2; Ma, D. Y.1; Liu, Q. H.1; Xu, S. H.1; Xiao, Z. Z.1; Lin, F.1,2; Xiao, Y. S.1; Li, J.1
刊名	JOURNAL OF FISH BIOLOGY
	2012-03-01
卷号	80 期号:3 页码:473-485
关键词	cytology gamete hybrid morphology oocyte spermatozoa
ISSN号	0022-1112
通讯作者	Li, J (reprint author), Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China.
英文摘要	The morphology of gametes and the fertilization biology of Japanese flounder Paralichthys olivaceus (Po), summer flounder Paralichthys dentatus (Pd) and their hybrids were examined. Multiple generations (two hybrids: Po female x Pd male (F1) and Pd female x Po male; two backcrosses: F1 female x Po male and F1 female x Pd male) were obtained by artificial insemination. Comparison of egg ultrastructure among Po, Pd and F1 showed the morphology of micropyle region and the distribution density of pores were species specific. There were c. 100-200 accessory openings around the micropyle in Po, but not in Pd and F1. The zona radiata thickness and number of parallel bands were similar between F1 and Po, which were different from Pd. Comparison of spermatozoa ultrastructure revealed a close relationship between Po and Pd. Cytologically, the six crosses obeyed normal fertilization and cleavage processes, and only one male pronucleus was observed in a fertilized egg, indicating a monospermic fertilization pattern. Analysis of the time distribution from fertilization to first cleavage revealed an obvious delay at pronucleus fusion in the Pd x Po cross. The delay might indicate some cytoplasmic-nuclear incompatibility during the process of fertilization.
学科主题	Fisheries ; Marine & Freshwater Biology
收录类别	SCI
原文出处	10.1111/j.1095-8649.2011.03146.x
语种	英语
WOS记录号	WOS:000300933900001
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12226]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Sui, J.,Ma, D. Y.,Liu, Q. H.,et al. Germ cells and fertilization differences among Japanese flounder Paralichthys olivaceus, summer flounder Paralichthys dentatus and their first and second generations[J]. JOURNAL OF FISH BIOLOGY,2012,80(3):473-485.
APA	Sui, J..,Ma, D. Y..,Liu, Q. H..,Xu, S. H..,Xiao, Z. Z..,...&Li, J..(2012).Germ cells and fertilization differences among Japanese flounder Paralichthys olivaceus, summer flounder Paralichthys dentatus and their first and second generations.JOURNAL OF FISH BIOLOGY,80(3),473-485.
MLA	Sui, J.,et al."Germ cells and fertilization differences among Japanese flounder Paralichthys olivaceus, summer flounder Paralichthys dentatus and their first and second generations".JOURNAL OF FISH BIOLOGY 80.3(2012):473-485.