Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)
	Jin, Weihua 1,2; Wang, Jing1 ; Ren, Sumei 3; Song, Ni 3; Zhang, Quanbin 1
刊名	MARINE DRUGS
	2012-10-01
卷号	10 期号:10 页码:2138-2152
关键词	ESI-CID-MS/MS fucoidan oligosaccharide Saccharina japonica
ISSN号	1660-3397
通讯作者	Zhang, QB (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
英文摘要	A fucoidan extracted from Saccharina japonica was fractionated by anion exchange chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and gel filtration chromatography while Fraction Y2 was fractionated by gel filtration chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1 -> 3 Man 1 -> 4 GlcA, Man 1 -> 3 GlcA 1 -> 4 GlcA, Fuc 1 -> 4 GlcA and Fuc 1 -> 3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6.
学科主题	Chemistry, Medicinal
收录类别	SCI
原文出处	10.3390/md10102138
语种	英语
WOS记录号	WOS:000310453600001
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12386]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean Univ China, Coll Med & Pharmaceut, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Jin, Weihua,Wang, Jing,Ren, Sumei,et al. Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)[J]. MARINE DRUGS,2012,10(10):2138-2152.
APA	Jin, Weihua,Wang, Jing,Ren, Sumei,Song, Ni,&Zhang, Quanbin.(2012).Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS).MARINE DRUGS,10(10),2138-2152.
MLA	Jin, Weihua,et al."Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)".MARINE DRUGS 10.10(2012):2138-2152.