Molecular cloning, expression pattern and antimicrobial activity of a new isoform of anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus

	Molecular cloning, expression pattern and antimicrobial activity of a new isoform of anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus
	Liu, Yuan 1,2; Cui, Zhaoxia 1; Li, Xihong 1,2; Song, Chengwen1,2 ; Li, Qianqian1,2 ; Wang, Shuangyan 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-07-01
卷号	33 期号:1 页码:85-91
关键词	Portunus trituberculatus Anti-lipopolysaccharide factor Expression analysis Antimicrobial activity
ISSN号	1050-4648
通讯作者	Cui, ZX (reprint author), Chinese Acad Sci, EMBL, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	A new isoform of anti-lipopolysaccharide factor (PtALF5) was cloned from eyestalk cDNA library of swimming crab Portunus trituberculatus. The full-length cDNA of PtALF5 was 1045 bp encoding 120 amino acids. PtALF5 shared lower amino acid similarity with other ALFs, yet it contained the conserved LPS-binding domain and was clearly member of the ALF family. The genomic fragment of PtALF5 contained two exons separated by one intron. Several tandem repeats were found in intron. The mRNA transcript of PtALF5 was predominantly expressed in the hemocytes but barely detectable in muscle. After challenge with Vibrio alginolyticus, a main pathogen causing high mortality in P. trituberculatus, the PtALF5 transcript in hemocytes showed a clear time-dependent response expression pattern with obvious decrease at 6 h and significant increase at 24 h. The recombinant PtALF5 protein revealed antimicrobial activity against Gram-negative bacteria V. alginolyticus and Pseudomonas aeruginosa, but did not inhibit the growth of the tested Gram-positive bacteria and fungus. These results together suggest that PtALF5 is a potent antibacterial protein against Gram-negative bacteria infection, and might function as a promising therapeutic agent in disease control of crab aquaculture. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2012.04.004
语种	英语
WOS记录号	WOS:000305378400011
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12363]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuan,Cui, Zhaoxia,Li, Xihong,et al. Molecular cloning, expression pattern and antimicrobial activity of a new isoform of anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(1):85-91.
APA	Liu, Yuan,Cui, Zhaoxia,Li, Xihong,Song, Chengwen,Li, Qianqian,&Wang, Shuangyan.(2012).Molecular cloning, expression pattern and antimicrobial activity of a new isoform of anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus.FISH & SHELLFISH IMMUNOLOGY,33(1),85-91.
MLA	Liu, Yuan,et al."Molecular cloning, expression pattern and antimicrobial activity of a new isoform of anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus".FISH & SHELLFISH IMMUNOLOGY 33.1(2012):85-91.