Isolation and characterization of microsatellites in Portunus trituberculatus

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Isolation and characterization of microsatellites in Portunus trituberculatus
	Cui, Zhaoxia; Liu, Yuan; Wang, Hongxia; Wu, Danhua; Luan, Weisha; Tan, Feng; Huang, Minde
刊名	CONSERVATION GENETICS RESOURCES
	2012-06-01
卷号	4 期号:2 页码:251-255
关键词	Portunus trituberculatus Microsatellites Polymorphism
ISSN号	1877-7252
通讯作者	Cui, ZX (reprint author), Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China.
英文摘要	Thirty polymorphic microsatellite markers for swimming crab P. trituberculatus are developed from EST sequences, genomic libraries and cross-species amplification in P. pelagicus, respectively. They are characterized using 24 P. trituberculatus individuals from Yingkou natural population. The number of alleles per locus range from 4 to 21 with a mean number of 10.83. The observed and expected heterozygosities range from 0.250 to 1.000 and from 0.365 to 0.945, respectively. Five loci departed significantly from HWE (P < 0.05). The genomic-SSRs and EST-SSRs show similar levels of polymorphism for both the average number of alleles (11 alleles vs. 10 alleles) and average PIC values (PIC = 0.780 vs. PIC = 0.776). These microsatellite loci from different sources will contribute to improve research on the genetic conservation of P. trituberculatus.
学科主题	Biodiversity Conservation ; Genetics & Heredity
收录类别	SCI
原文出处	10.1007/s12686-011-9518-0
语种	英语
WOS记录号	WOS:000303536400010
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12354]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Cui, Zhaoxia,Liu, Yuan,Wang, Hongxia,et al. Isolation and characterization of microsatellites in Portunus trituberculatus[J]. CONSERVATION GENETICS RESOURCES,2012,4(2):251-255.
APA	Cui, Zhaoxia.,Liu, Yuan.,Wang, Hongxia.,Wu, Danhua.,Luan, Weisha.,...&Huang, Minde.(2012).Isolation and characterization of microsatellites in Portunus trituberculatus.CONSERVATION GENETICS RESOURCES,4(2),251-255.
MLA	Cui, Zhaoxia,et al."Isolation and characterization of microsatellites in Portunus trituberculatus".CONSERVATION GENETICS RESOURCES 4.2(2012):251-255.