Induction of Apoptosis, G(0)/G(1) Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus

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	Induction of Apoptosis, G(0)/G(1) Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus
	Liu, Ming 1; Zhao, Xiangzhong 2; Zhao, Jin 1; Xiao, Lin 1; Liu, Haizhou 1; Wang, Cuicui 1; Cheng, Linyou 1; Wu, Ning 1; Lin, Xiukun 1
刊名	MARINE DRUGS
	2012-11-01
卷号	10 期号:11 页码:2596-2607
关键词	Meretrix meretrix Linnaeus Mere15 apoptosis anticancer
ISSN号	1660-3397
通讯作者	Lin, XK (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Mere15 is a novel polypeptide from Meretrix meretrix Linnaeus with cytotoxicity in solid cancer cells. In this study, we investigated its activity on human K562 chronic myelogenous leukemia cells. Mere15 inhibited the growth of K562 cells with IC50 values of 38.2 mu g/mL. Mere15 also caused concentration dependent induction of apoptosis, with overproduction of reactive oxygen species and loss of mitochondrial membrane potential. Moreover, Mere15 arrested cell cycle progression at G(0)/G(1) phase of K562 cells in a concentration dependent manner. In addition, Mere15 caused the disassembly of the microtubule cytoskeleton in K562 cells and inhibited the polymerization of tubulin in a cell free system via interaction with tubulin. We concluded that Mere15 was cytotoxic to K562 leukemia cells and the cytotoxicity was related to the apoptosis induction, cell cycle arrest and microtubule disassembly. These results implied that Merer15 was a broad spectrum anticancer polypeptide, not only cytotoxic to various solid cancer cells but also to the chronic myelogenous leukemia cells. Mere15 may have therapeutic potential for the treatment of leukemia.
学科主题	Chemistry, Medicinal
收录类别	SCI
原文出处	10.3390/md10112596
语种	英语
WOS记录号	WOS:000311425800016
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12353]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Shandong Acad Med Sci, Key Lab Rare Dis Shandong Prov, Inst Basic Med, Jinan 250062, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ming,Zhao, Xiangzhong,Zhao, Jin,et al. Induction of Apoptosis, G(0)/G(1) Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus[J]. MARINE DRUGS,2012,10(11):2596-2607.
APA	Liu, Ming.,Zhao, Xiangzhong.,Zhao, Jin.,Xiao, Lin.,Liu, Haizhou.,...&Lin, Xiukun.(2012).Induction of Apoptosis, G(0)/G(1) Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus.MARINE DRUGS,10(11),2596-2607.
MLA	Liu, Ming,et al."Induction of Apoptosis, G(0)/G(1) Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus".MARINE DRUGS 10.11(2012):2596-2607.