Improvement of hydrogen production by transposon-mutagenized strain of Pantoea agglomerans BH18

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	Improvement of hydrogen production by transposon-mutagenized strain of Pantoea agglomerans BH18
	Liu, Hongyan 1; Wang, Guangce 1,2; Zhu, Daling 1; Pan, Guanghua 1
刊名	INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
	2012-05-01
卷号	37 期号:10 页码:8282-8287
关键词	Transposon Tn7 Hydrogen production Pantoea agglomerans BH18
ISSN号	0360-3199
通讯作者	Wang, GC (reprint author), Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China.
英文摘要	Pantoea agglomerans BH18, isolated from mangrove sludge, could produce hydrogen under marine culture condition. To improve the hydrogen-producing capacity of this strain, we constructed a stable transposon-mutagenized library of P. agglomerans BH18. A Tn7-based transposon was randomly inserted into genomic DNA of P. agglomerans BH18. Mutants were identified by kanamycin resistance and amplification of the inserted transposon sequences. A transposon mutant, named as strain TB212, was screened for the highest hydrogen production ability. The total volume of hydrogen gas evolved by this mutant strain TB212 was 60% higher than that of the wild type. The mutant strain TB212 was able to produce hydrogen over a wide range of initial pH from 5.0 to 10.0, with an optimum initial pH of 7.0, and hydrogen production was 2.52 +/- 0.02 mol H-2/mol glucose (mean +/- SE.) under marine culture condition. The mutant strain TB212 could produce hydrogen at the salt concentration from 3 to 6%. It was concluded that the transposon-mutagenized library may be a useful tool for investigation of high efficiency hydrogen-producing bacteria. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
学科主题	Chemistry, Physical ; Electrochemistry ; Energy & Fuels
收录类别	SCI
原文出处	10.1016/j.ijhydene.2012.02.065
语种	英语
WOS记录号	WOS:000305040400019
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12352]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin Key Lab Marine Resources & Chem, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hongyan,Wang, Guangce,Zhu, Daling,et al. Improvement of hydrogen production by transposon-mutagenized strain of Pantoea agglomerans BH18[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2012,37(10):8282-8287.
APA	Liu, Hongyan,Wang, Guangce,Zhu, Daling,&Pan, Guanghua.(2012).Improvement of hydrogen production by transposon-mutagenized strain of Pantoea agglomerans BH18.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,37(10),8282-8287.
MLA	Liu, Hongyan,et al."Improvement of hydrogen production by transposon-mutagenized strain of Pantoea agglomerans BH18".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 37.10(2012):8282-8287.