Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad

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	Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad
	Wu, Ning 1,2; Yu, Ai-Bing 3; Zhu, Hua-Bin 2; Lin, Xiu-Kun 1,2
刊名	JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
	2012
页码	343891
关键词	SEX-DETERMINING GENE PRE-SERTOLI CELLS TESTIS DEVELOPMENT IN-VIVO MAMMALIAN-CELLS PLASMID DNA EXPRESSION MICE WT1 SOX9
ISSN号	1110-7243
通讯作者	Lin, XK (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Delivering siRNA or shRNA into the developing embryos is still a main challenge to use of RNAi in mammalian systems. Here we analyze several factors influencing RNAi-mediated silencing of Sry gene, which is a tightly controlled spatiotemporal expressed gene and only shortly expressed in developing mouse embryo gonad. A Sry gene-specific shRNAs expression vector (pSilencer4.1/Sry565) was constructed. The shRNA constructs were mixed with polyethylenimines (PEIs) to form a complex and then injected into pregnant mice though tail vein. Our results showed that Sry gene was downregulated significantly in developing embryos. Further study revealed that knocking-down of Sry expression resulted in feminization of gonad development in mouse embryos and the expression level of Sox9 and Wt1 gene was also significantly changed by downregulation of Sry. The transfection efficiency is associated with the amount of plasmid DNA injection, injection time, injection speed, and volume. Our studies suggest that transplacental RNAi could be implemented by tail vein injection of plasmid vector into pregnant mice.
学科主题	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
收录类别	SCI
原文出处	10.1155/2012/343891
语种	英语
WOS记录号	WOS:000301372700001
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12317]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100094, Peoples R China 3.Yangzhou Univ, Anim Nutr Lab, Grad Sch Anim Sci & Technol, Yangzhou 225009, Peoples R China
推荐引用方式 GB/T 7714	Wu, Ning,Yu, Ai-Bing,Zhu, Hua-Bin,et al. Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad[J]. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,2012:343891.
APA	Wu, Ning,Yu, Ai-Bing,Zhu, Hua-Bin,&Lin, Xiu-Kun.(2012).Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad.JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,343891.
MLA	Wu, Ning,et al."Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad".JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2012):343891.