CHARACTERIZATION OF THE ALTERNATIVE OXIDASE GENE IN PORPHYRA YEZOENSIS (RHODOPHYTA) AND CYANIDE-RESISTANT RESPIRATION ANALYSIS

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	CHARACTERIZATION OF THE ALTERNATIVE OXIDASE GENE IN PORPHYRA YEZOENSIS (RHODOPHYTA) AND CYANIDE-RESISTANT RESPIRATION ANALYSIS
	Zhang, Bao Y.1; He, Lin W.1; Jia, Zhao J.2; Wang, Guang C.1; Peng, Guang 1
刊名	JOURNAL OF PHYCOLOGY
	2012-06-01
卷号	48 期号:3 页码:657-663
关键词	alternative oxidase alternative respiration pathway mRNA expression noninvasive microsensing system Porphyra yezoensis
ISSN号	0022-3646
通讯作者	Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
英文摘要	The full-length cDNA of the alternative oxidase (AOX) gene from Porphyra yezoensis Ueda (PyAOX) [currently assigned as Pyropia yezoensis (Ueda) M. S. Hwang et H. G. Choi ()] an ancient member of the Rhodphyta, was cloned by electronic cloning, rapid amplification of cDNA ends (RACE), and reverse transcription PCR. The nucleotide sequence of PyAOX consists of 1,650 bp, including a 5' untranslated region (UTR) of 170 bp, a 3' UTR of 148 bp, and an open reading frame (ORF) of 1,332 bp that can be translated into a 443-amino-acid residue with a molecular mass of 47.33 kDa and a putative isoelectric point (pI) of 9.71. The putative amino acids had 50%61% identity with AOX genes in Eukaryota and higher plants and had AOX-like characteristics. The expression of PyAOX mRNA in different stages of the life cycle, conchospores, filamentous thalli (conchocelis stage), and leafy thalli, was detected by real-time quantitative PCR (qPCR). The highest level of expression, which was observed in filamentous thalli, was three times higher than that observed in leafy thalli. The next highest level, which was observed in the conchospores, was twice as high as that observed in leafy thalli. We showed that an alternative respiration pathway existed in P. yezoensis with a noninvasive microsensing system. The contribution of the alternative pathway to total respiration in filamentous thalli was greater than that in leafy thalli. This result was consistent with the level of AOX gene expression observed in different stages of the life cycle.
学科主题	Plant Sciences ; Marine & Freshwater Biology
收录类别	SCI
原文出处	10.1111/j.1529-8817.2012.01129.x
语种	英语
WOS记录号	WOS:000304810200017
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12298]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Bao Y.,He, Lin W.,Jia, Zhao J.,et al. CHARACTERIZATION OF THE ALTERNATIVE OXIDASE GENE IN PORPHYRA YEZOENSIS (RHODOPHYTA) AND CYANIDE-RESISTANT RESPIRATION ANALYSIS[J]. JOURNAL OF PHYCOLOGY,2012,48(3):657-663.
APA	Zhang, Bao Y.,He, Lin W.,Jia, Zhao J.,Wang, Guang C.,&Peng, Guang.(2012).CHARACTERIZATION OF THE ALTERNATIVE OXIDASE GENE IN PORPHYRA YEZOENSIS (RHODOPHYTA) AND CYANIDE-RESISTANT RESPIRATION ANALYSIS.JOURNAL OF PHYCOLOGY,48(3),657-663.
MLA	Zhang, Bao Y.,et al."CHARACTERIZATION OF THE ALTERNATIVE OXIDASE GENE IN PORPHYRA YEZOENSIS (RHODOPHYTA) AND CYANIDE-RESISTANT RESPIRATION ANALYSIS".JOURNAL OF PHYCOLOGY 48.3(2012):657-663.