Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China
	Zhang, Min; Xiao, Zhi-zhong; Hu, Yong-hua; Sun, Li
刊名	AQUACULTURE RESEARCH
	2012-03-01
卷号	43 期号:4 页码:556-564
关键词	iridovirus megalocytivirus Oplegnathus fasciatus (Temminck & Schlege) Scophthalmus maximus L
ISSN号	1355-557X
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Megalocytivirus belongs to the Iridoviridae family and is known to affect finfish. Megalocytivirus epizootics have been reported to occur in several cultured fish species in China; however, no megalocytivirus associated with rock bream, Oplegnathus fasciatus (Temminck & Schlege), has been documented. In this study, we characterized for the first time a megalocytivirus, rock bream iridovirus (RBIV)-C1, detected in cultured rock bream in a fish farm in China that had been inflicted with a high-mortality disease outbreak. Sequence analysis of three conserved genes showed that RBIV-C1 shares over 90% overall identities with a number of known megalocytiviruses. Electron microscopic examination revealed RBIV-C1 as hexagonal particles similar to those reported for megalocytiviruses. In vivo infection study indicated that, following inoculation into rock bream, RBIV-C1 induced 100% mortality and upregulated the expression of Mx, IL-1 beta and IL-8. The infected fish exhibited pathological signs similar to those observed in naturally diseased fish. Furthermore, studies in a turbot (Scophthalmus maximus L.) model indicated that RBIV-C1 induced acute infection in turbot that led to 100% mortality. These results indicate that RBIV-C1 is highly virulent to rock bream as well as turbot and that RBIV-C1 is closely related to a number of previously reported megalocytivirus and likely a genetic variant of the latter.
学科主题	Fisheries
收录类别	SCI
原文出处	10.1111/j.1365-2109.2011.02861.x
语种	英语
WOS记录号	WOS:000301226600009
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12296]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Min,Xiao, Zhi-zhong,Hu, Yong-hua,et al. Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China[J]. AQUACULTURE RESEARCH,2012,43(4):556-564.
APA	Zhang, Min,Xiao, Zhi-zhong,Hu, Yong-hua,&Sun, Li.(2012).Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China.AQUACULTURE RESEARCH,43(4),556-564.
MLA	Zhang, Min,et al."Characterization of a megalocytivirus from cultured rock bream, Oplegnathus fasciatus (Temminck & Schlege), in China".AQUACULTURE RESEARCH 43.4(2012):556-564.