Association of CfLGBP gene polymorphism with disease susceptibility/resistance of Zhikong scallop (Chlamys farreri) to Listonella anguillarum

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	Association of CfLGBP gene polymorphism with disease susceptibility/resistance of Zhikong scallop (Chlamys farreri) to Listonella anguillarum
	Siva, Vinu S.1,2; Yang, Chuanyan 1,2; Yang, Jialong 1,2; Wang, Lingling 1; Wang, Leilei 1,2; Zhou, Zhi 1,2; Qiu, Limei 1; Song, Linsheng 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-06-01
卷号	32 期号:6 页码:1117-1123
关键词	Chlamys farreri Lipopolysaccharide and beta-1 3-glucan binding protein Single nucleotide polymorphism Disease resistance Disease susceptibility
ISSN号	1050-4648
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Lipopolysaccharide and beta-1, 3-glucan binding protein (LGBP) is a pattern recognition receptor (PRR) recognizing and binding both LPS and beta-1, 3-glucan, playing important roles in innate immunity. In the present study, the single nucleotide polymorphisms (SNF's) were assessed in LGBP gene from scallop Chlamys farreri (designated CfLGBP), and eight SNPs were found in its potential LPS and glucanase binding motif. The locus +7679 with the transition of G-A, which produced an amino acid substitution at codon 360 from a non polar Glycine to polar Serine, was selected to inspect their association with disease resistance/susceptibility to Listonella anguillarum. Three genotypes G/G, G/A and A/A, were revealed at locus +7679, and their frequencies were 89.7%, 7.7% and 2.6% in the resistant stock, while 63.2%, 34.2% and 2.6% in the susceptible stock, respectively. The frequency of genotypes G/G and G/A were significantly different (P < 0.05) between the two stocks. The pathogen-associated molecular patterns (PAMP) binding activity of two recombinant proteins, rCfLGBP (G) with G variant at locus +7679 and rCfLGBP (S) with A variant at locus +7679, were elucidated by ELISA assay. The binding affinities of both LPS and beta-glucan binding affinity were varied in a dose-dependent manner, where the binding affinity of rCfLGBP (G) was significantly higher than that of rCfLGBP (S) (P < 0.05). The results collectively suggested that the polymorphism of +7679 G/G in CfLGBP possibly enhances the binding activity of LPS and beta-glucan, and was associated to disease resistance of scallop against L anguillarum, which could be a potential marker applied in future selection of scallop with enhanced resistance to L anguillarum. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2012.03.017
语种	英语
WOS记录号	WOS:000304511800021
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12288]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Siva, Vinu S.,Yang, Chuanyan,Yang, Jialong,et al. Association of CfLGBP gene polymorphism with disease susceptibility/resistance of Zhikong scallop (Chlamys farreri) to Listonella anguillarum[J]. FISH & SHELLFISH IMMUNOLOGY,2012,32(6):1117-1123.
APA	Siva, Vinu S..,Yang, Chuanyan.,Yang, Jialong.,Wang, Lingling.,Wang, Leilei.,...&Song, Linsheng.(2012).Association of CfLGBP gene polymorphism with disease susceptibility/resistance of Zhikong scallop (Chlamys farreri) to Listonella anguillarum.FISH & SHELLFISH IMMUNOLOGY,32(6),1117-1123.
MLA	Siva, Vinu S.,et al."Association of CfLGBP gene polymorphism with disease susceptibility/resistance of Zhikong scallop (Chlamys farreri) to Listonella anguillarum".FISH & SHELLFISH IMMUNOLOGY 32.6(2012):1117-1123.