CsCXCe1: A novel Cynoglossus semilaevis CXC chemokine that functions as a chemoattractant and an immunomodulator for peripheral blood leukocytes

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	CsCXCe1: A novel Cynoglossus semilaevis CXC chemokine that functions as a chemoattractant and an immunomodulator for peripheral blood leukocytes
	Li, Yong-xin 1,2; Hu, Yong-hua 1; sun, Jin-sheng 2,3; Sun, Li 1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2012-05-01
卷号	37 期号:1 页码:55-64
关键词	CXC chemokine Cynoglossus semilaevis Chemotaxis Immunoregulation Immunomodulator
ISSN号	0145-305X
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, PR, Peoples R China.
英文摘要	Chemokines are small cytokines that, based on their structural differences, are classified into four groups, one of which is called CXC chemokines. In this study, we identified a CXC chemokine, CsCXCe1, from half-smooth tongue sole (Cynoglossus semilaevis) and analyzed its function. The deduced amino acid sequence of CsCXCe1 contains 115 residues and is phylogenetically distinct from known CXC chemokines. CsCXCe1 possesses the conserved RCXC motif in the form of RCWC but lacks the ELR sequence that is found in some CXC chemokines. Expression of CsCXCe1 as determined by quantitative real time RT-PCR occurred abundantly in immune organs and was upregulated by bacterial and viral infection in time dependent manners. Purified recombinant CsCXCe1 (rCsCXCe1) exhibited comparable chemotactic activities against tongue sole and turbot (Scophthalmus maximus) peripheral blood leukocytes (PBL). Microscopic analysis identified lymphocytes as the major cellular population in PBL that responds to rCsCXCe1. Mutational study showed that when the two cysteine residues in the RCWC motif of CsCXCe1 were substituted by serine, the chemoattractive activity of CsCXCe1 was completely lost. Further study showed that treatment of PBL with rCsCXCe1 (i) stimulated cellular proliferation and respiratory burst activity. (ii) upregulated the expression of a wide spectrum of immune relevant genes, and (iii) enhanced cellular resistance against bacterial infection. Taken together, these results indicate that CsCXCe1 is likely a new type of CXC chemokine that exerts chemotactic and immunostimulatory effects on PBL. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题	Immunology ; Zoology
收录类别	SCI
原文出处	10.1016/j.dci.2011.12.007
语种	英语
WOS记录号	WOS:000304024700007
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12263]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, PR, Peoples R China 2.Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, PR, Peoples R China 3.Tianjin Aquaculture Dis Prevent & Treatment Ctr, Tianjin 300221, PR, Peoples R China
推荐引用方式 GB/T 7714	Li, Yong-xin,Hu, Yong-hua,sun, Jin-sheng,et al. CsCXCe1: A novel Cynoglossus semilaevis CXC chemokine that functions as a chemoattractant and an immunomodulator for peripheral blood leukocytes[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,37(1):55-64.
APA	Li, Yong-xin,Hu, Yong-hua,sun, Jin-sheng,&Sun, Li.(2012).CsCXCe1: A novel Cynoglossus semilaevis CXC chemokine that functions as a chemoattractant and an immunomodulator for peripheral blood leukocytes.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,37(1),55-64.
MLA	Li, Yong-xin,et al."CsCXCe1: A novel Cynoglossus semilaevis CXC chemokine that functions as a chemoattractant and an immunomodulator for peripheral blood leukocytes".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 37.1(2012):55-64.