A strategy for practical authentication of medicinal plants in traditional Chinese medicine prescription, paeony root in ShaoYao-GanCao decoction as a case study

doi:10.1002/jssc.202100028

	A strategy for practical authentication of medicinal plants in traditional Chinese medicine prescription, paeony root in ShaoYao-GanCao decoction as a case study
	Cheng, Mengzhen 1,2; Yao, Changliang 2; Li, Yun 2; Li, Zhenwei 2; Li, Haojv 2; Yao, Shuai 2; Qu, Hua 1,2; Li, Jiayuan 2; Wei, Wenlong 2; Zhang, Jianqing 2
刊名	JOURNAL OF SEPARATION SCIENCE
	2021-05-11
页码	11
关键词	authentication chemical markers paeony root selected ion monitoring traditional Chinese medicine
ISSN号	1615-9306
DOI	10.1002/jssc.202100028
通讯作者	Guo, De-an(daguo@simm.ac.cn)
英文摘要	Authentication of Chinese medicine materials in prescriptions is extremely difficult due to the complicated chemical matrix. A strategy integrating in-depth profiling, chemical marker selection, and selected detection was established and exemplarily applied to authenticate paeony root in ShaoYao-GanCao decoction. First, an ultra-performance liquid chromatography/linear trap quadrupole-Orbitrap method was developed to probe the chemical compositions of the decoction. Second, 20 batches of decoctions prepared from white paeony root and red paeony root were compared by a metabolomics method, and multistep chemometrics analysis distinguished the chemical markers. Third, an ultra-performance liquid chromatography/QDa-selected ion monitoring method was developed to authenticate the paeony root in decoctions. As a result, 161 compounds were characterized, including 84 triterpene saponins, 42 flavonoids, and 10 monoterpenes. Four chemical markers and paeoniflorin were successfully screened out as chemical markers for white paeony root. The selected ion monitoring method easily differentiated authentic decoction (prepared from white paeony root) from fraud decoction (prepared from red paeony root) by monitoring the above five chemical markers. In conclusion, the strategy was proved effective in authentication of paeony root in ShaoYao-GanCao decoction, and it can also be applied to authenticate other Chinese medicine materials in prescriptions, which will greatly avail the quality enhancement of prescriptions.
资助项目	National Key R&D Program of China[2019YFC1711000] ; National Key R&D Program of China[2019YFC1711400] ; National Key R&D Program of China[2018YFC1707900] ; National Natural Science Foundation of China[82003938] ; National Traditional Chinese Medicine Leading Talents Support Program[2018]
WOS关键词	ION ; IDENTIFICATION ; MARKERS
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000648875700001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296535]
专题	中国科学院上海药物研究所
通讯作者	Guo, De-an
作者单位	1.Nanjing Univ Chinese Med, Sch Chinese Materia Med, Nanjing, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Haike Rd 501, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Mengzhen,Yao, Changliang,Li, Yun,et al. A strategy for practical authentication of medicinal plants in traditional Chinese medicine prescription, paeony root in ShaoYao-GanCao decoction as a case study[J]. JOURNAL OF SEPARATION SCIENCE,2021:11.
APA	Cheng, Mengzhen.,Yao, Changliang.,Li, Yun.,Li, Zhenwei.,Li, Haojv.,...&Guo, De-an.(2021).A strategy for practical authentication of medicinal plants in traditional Chinese medicine prescription, paeony root in ShaoYao-GanCao decoction as a case study.JOURNAL OF SEPARATION SCIENCE,11.
MLA	Cheng, Mengzhen,et al."A strategy for practical authentication of medicinal plants in traditional Chinese medicine prescription, paeony root in ShaoYao-GanCao decoction as a case study".JOURNAL OF SEPARATION SCIENCE (2021):11.